Erdr1 suppresses murine melanoma growth via regulation of apoptosis

Joohyun Lee, Min Kyung Jung, Hyun Jeong Park, Kyung Eun Kim, Dae Ho Cho

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Melanoma, one of the aggressive cancers, is known to be resistant to chemotherapy. Because of its aggressive nature, effectively inducing apoptosis is necessary to treat melanoma. Erythroid differentiation regulator 1 (Erdr1) is known to be a stress-related survival factor exhibiting anti-cancer effects in several cancers. However, little is known about the functions and underlying mechanisms of Erdr1 so far. To demonstrate the effect of Erdr1 in melanoma apoptosis, recombinant murine Erdr1 was injected into mice implanted with B16F10 melanoma cells. In vivo tumor growth was significantly inhibited in mice injected with Erdr1 compared to the control. In addition, the tumor from Erdr1-injected mice showed an increased level of apoptosis. Accordingly, apoptosis-regulating factors including anti-apoptotic marker Bcl-2 and pro-apoptotic marker Bax in the tumor tissues were examined. As expected, the decreased level of Bcl-2 and increased level of Bax were detected in tumors within the mice injected with Erdr1. Based on the in vivo study, the role of Erdr1 in tumor apoptosis was further tested by incubating it with cells of the murine melanoma cell line B16F10. Erdr1-induced apoptosis in B16F10 cells was observed. Additionally, Erdr1 downregulated STAT3 activity, inhibiting apoptosis via regulation of the Bcl-2 family. Overall, data demonstrate that Erdr1 induced murine melanoma apoptosis through the regulation of Bcl-2 and Bax. These findings suggest that Erdr1 is a novel regulator of apoptosis in melanoma.

Original languageEnglish
Article number107
JournalInternational journal of molecular sciences
Volume17
Issue number1
DOIs
Publication statusPublished - 2016 Jan 14
Externally publishedYes

Fingerprint

regulators
apoptosis
Cell death
Melanoma
Apoptosis
Growth
Tumors
Neoplasms
tumors
mice
cancer
markers
Chemotherapy
cells
Down-Regulation
Cells
chemotherapy
Tissue
cultured cells
Drug Therapy

Keywords

  • Apoptosis
  • Bax
  • Bcl-2
  • Erdr1
  • Melanoma

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Erdr1 suppresses murine melanoma growth via regulation of apoptosis. / Lee, Joohyun; Jung, Min Kyung; Park, Hyun Jeong; Kim, Kyung Eun; Cho, Dae Ho.

In: International journal of molecular sciences, Vol. 17, No. 1, 107, 14.01.2016.

Research output: Contribution to journalArticle

Lee, Joohyun ; Jung, Min Kyung ; Park, Hyun Jeong ; Kim, Kyung Eun ; Cho, Dae Ho. / Erdr1 suppresses murine melanoma growth via regulation of apoptosis. In: International journal of molecular sciences. 2016 ; Vol. 17, No. 1.
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