Erythroid differentiation regulator 1 strengthens TCR signaling in thymocytes by modulating calcium flux

Myun Soo Kim, Sora Lee, Su Jin Jung, Sunyoung Park, Kyung Eun Kim, Tae Sung Kim, Hyun Jeong Park, Dae Ho Cho

Research output: Contribution to journalArticle

Abstract

Erythroid differentiation regulator 1 (Erdr1) has been identified as a stromal survival factor released under stressful conditions. Previously, Erdr1 was reported to be expressed highly in thymus, but roles of Erdr1 in thymus were not known. Here, the effects of Erdr1 on T cell development were investigated. The expression of Erdr1 was higher in thymus than bone marrow and Erdr1 was detected in both the cortex and medulla of thymus. Erdr1 treatment significantly induced the expression of activation marker, CD69, from thymocytes in the presence of TCR stimuli in vitro and the induction was dependent on increased Ca2+ influx. In addition, in vivo administration of Erdr1 resulted in significant increase of total and positive selected thymocyte numbers, particularly in the number of CD3TCRhiCD69+ DP thymocytes. Taken together, our results show that Erdr1 enhances the strength of TCR signaling and cellularity of thymocytes by amplifying Ca2+ influx in thymocytes receiving TCR signals.

Original languageEnglish
JournalCellular Immunology
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Thymocytes
Thymus Gland
Calcium
Bone Marrow
T-Lymphocytes

Keywords

  • Calcium flux
  • Erdr1
  • TCR
  • Thymocyte

ASJC Scopus subject areas

  • Immunology

Cite this

Erythroid differentiation regulator 1 strengthens TCR signaling in thymocytes by modulating calcium flux. / Kim, Myun Soo; Lee, Sora; Jung, Su Jin; Park, Sunyoung; Kim, Kyung Eun; Kim, Tae Sung; Park, Hyun Jeong; Cho, Dae Ho.

In: Cellular Immunology, 01.01.2018.

Research output: Contribution to journalArticle

Kim, Myun Soo ; Lee, Sora ; Jung, Su Jin ; Park, Sunyoung ; Kim, Kyung Eun ; Kim, Tae Sung ; Park, Hyun Jeong ; Cho, Dae Ho. / Erythroid differentiation regulator 1 strengthens TCR signaling in thymocytes by modulating calcium flux. In: Cellular Immunology. 2018.
@article{3cbccf80044a4283a1ad5fd0b037e6e7,
title = "Erythroid differentiation regulator 1 strengthens TCR signaling in thymocytes by modulating calcium flux",
abstract = "Erythroid differentiation regulator 1 (Erdr1) has been identified as a stromal survival factor released under stressful conditions. Previously, Erdr1 was reported to be expressed highly in thymus, but roles of Erdr1 in thymus were not known. Here, the effects of Erdr1 on T cell development were investigated. The expression of Erdr1 was higher in thymus than bone marrow and Erdr1 was detected in both the cortex and medulla of thymus. Erdr1 treatment significantly induced the expression of activation marker, CD69, from thymocytes in the presence of TCR stimuli in vitro and the induction was dependent on increased Ca2+ influx. In addition, in vivo administration of Erdr1 resulted in significant increase of total and positive selected thymocyte numbers, particularly in the number of CD3TCRhiCD69+ DP thymocytes. Taken together, our results show that Erdr1 enhances the strength of TCR signaling and cellularity of thymocytes by amplifying Ca2+ influx in thymocytes receiving TCR signals.",
keywords = "Calcium flux, Erdr1, TCR, Thymocyte",
author = "Kim, {Myun Soo} and Sora Lee and Jung, {Su Jin} and Sunyoung Park and Kim, {Kyung Eun} and Kim, {Tae Sung} and Park, {Hyun Jeong} and Cho, {Dae Ho}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.cellimm.2018.12.004",
language = "English",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Erythroid differentiation regulator 1 strengthens TCR signaling in thymocytes by modulating calcium flux

AU - Kim, Myun Soo

AU - Lee, Sora

AU - Jung, Su Jin

AU - Park, Sunyoung

AU - Kim, Kyung Eun

AU - Kim, Tae Sung

AU - Park, Hyun Jeong

AU - Cho, Dae Ho

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Erythroid differentiation regulator 1 (Erdr1) has been identified as a stromal survival factor released under stressful conditions. Previously, Erdr1 was reported to be expressed highly in thymus, but roles of Erdr1 in thymus were not known. Here, the effects of Erdr1 on T cell development were investigated. The expression of Erdr1 was higher in thymus than bone marrow and Erdr1 was detected in both the cortex and medulla of thymus. Erdr1 treatment significantly induced the expression of activation marker, CD69, from thymocytes in the presence of TCR stimuli in vitro and the induction was dependent on increased Ca2+ influx. In addition, in vivo administration of Erdr1 resulted in significant increase of total and positive selected thymocyte numbers, particularly in the number of CD3TCRhiCD69+ DP thymocytes. Taken together, our results show that Erdr1 enhances the strength of TCR signaling and cellularity of thymocytes by amplifying Ca2+ influx in thymocytes receiving TCR signals.

AB - Erythroid differentiation regulator 1 (Erdr1) has been identified as a stromal survival factor released under stressful conditions. Previously, Erdr1 was reported to be expressed highly in thymus, but roles of Erdr1 in thymus were not known. Here, the effects of Erdr1 on T cell development were investigated. The expression of Erdr1 was higher in thymus than bone marrow and Erdr1 was detected in both the cortex and medulla of thymus. Erdr1 treatment significantly induced the expression of activation marker, CD69, from thymocytes in the presence of TCR stimuli in vitro and the induction was dependent on increased Ca2+ influx. In addition, in vivo administration of Erdr1 resulted in significant increase of total and positive selected thymocyte numbers, particularly in the number of CD3TCRhiCD69+ DP thymocytes. Taken together, our results show that Erdr1 enhances the strength of TCR signaling and cellularity of thymocytes by amplifying Ca2+ influx in thymocytes receiving TCR signals.

KW - Calcium flux

KW - Erdr1

KW - TCR

KW - Thymocyte

UR - http://www.scopus.com/inward/record.url?scp=85058017512&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058017512&partnerID=8YFLogxK

U2 - 10.1016/j.cellimm.2018.12.004

DO - 10.1016/j.cellimm.2018.12.004

M3 - Article

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

ER -