Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element

Jae Hong Kim; Hee Jin Kwack; Sung E. Choi; Byung Chul Kim; Yong Sun Kim; Il Jun Kang; Chandra C. Kumar

doi:10.1016/S0014-5793(97)00249-4

Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element

Jae Hong Kim, Hee Jin Kwack, Sung E. Choi, Byung Chul Kim, Yong Sun Kim, Il Jun Kang, Chandra C. Kumar

Department of Biotechnology

Research output: Contribution to journal › Article

14 Citations (Scopus)

Abstract

In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H₂O₂ stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A₂ (PLA₂) in the H₂O₂-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA₂, blocked H₂O₂-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A₂' in the signaling pathway of H₂O₂ to SRE.

Original language	English
Pages (from-to)	93-96
Number of pages	4
Journal	FEBS Letters
Volume	406
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(97)00249-4
Publication status	Published - 1997 Apr 7

Keywords

Arachidonic acid
Hydrogen peroxide
Phospholipase A
Rac GTPase
Serum response element
c-fos

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element",

abstract = "In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.",

keywords = "Arachidonic acid, Hydrogen peroxide, Phospholipase A, Rac GTPase, Serum response element, c-fos",

author = "Kim, {Jae Hong} and Kwack, {Hee Jin} and Choi, {Sung E.} and Kim, {Byung Chul} and Kim, {Yong Sun} and Kang, {Il Jun} and Kumar, {Chandra C.}",

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AU - Kim, Jae Hong

AU - Kwack, Hee Jin

AU - Choi, Sung E.

AU - Kim, Byung Chul

AU - Kim, Yong Sun

AU - Kang, Il Jun

AU - Kumar, Chandra C.

PY - 1997/4/7

Y1 - 1997/4/7

N2 - In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.

AB - In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.

KW - Arachidonic acid

KW - Hydrogen peroxide

KW - Phospholipase A

KW - Rac GTPase

KW - Serum response element

KW - c-fos

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