Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element

Jae-Hong Kim, Hee J. Kwack, Sung E. Choi, Byung Chul Kim, Yong S. Kim, Il J. Kang, Chandra C. Kumar

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.

Original languageEnglish
Pages (from-to)93-96
Number of pages4
JournalFEBS Letters
Volume406
Issue number1-2
DOIs
Publication statusPublished - 1997 Apr 7
Externally publishedYes

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Keywords

  • Arachidonic acid
  • c-fos
  • Hydrogen peroxide
  • Phospholipase A
  • Rac GTPase
  • Serum response element

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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