Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element

Jae Hong Kim; Hee Jin Kwack; Sung E. Choi; Byung Chul Kim; Yong Sun Kim; Il Jun Kang; Chandra C. Kumar

doi:10.1016/S0014-5793(97)00249-4

Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element

Jae Hong Kim, Hee Jin Kwack, Sung E. Choi, Byung Chul Kim, Yong Sun Kim, Il Jun Kang, Chandra C. Kumar

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H₂O₂ stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A₂ (PLA₂) in the H₂O₂-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA₂, blocked H₂O₂-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A₂' in the signaling pathway of H₂O₂ to SRE.

Original language	English
Pages (from-to)	93-96
Number of pages	4
Journal	FEBS Letters
Volume	406
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(97)00249-4
Publication status	Published - 1997 Apr 7
Externally published	Yes

Keywords

Arachidonic acid
Hydrogen peroxide
Phospholipase A
Rac GTPase
Serum response element
c-fos

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element",

abstract = "In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.",

keywords = "Arachidonic acid, Hydrogen peroxide, Phospholipase A, Rac GTPase, Serum response element, c-fos",

author = "Kim, {Jae Hong} and Kwack, {Hee Jin} and Choi, {Sung E.} and Kim, {Byung Chul} and Kim, {Yong Sun} and Kang, {Il Jun} and Kumar, {Chandra C.}",

note = "Funding Information: This work was supported by grant from Hallym Academy of Science, Hallym University, 1996 (to I.J. Kang). We wish to thank Dr. Ron. Prywes (Columbia University, USA) for providing us with SRE-luciferase plasmids. We also wish to thank Dr. Allan Hall (University College, London, UK) for providing us pEXV-Rac plasmid.",

year = "1997",

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doi = "10.1016/S0014-5793(97)00249-4",

language = "English",

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pages = "93--96",

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T1 - Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element

AU - Kim, Jae Hong

AU - Kwack, Hee Jin

AU - Choi, Sung E.

AU - Kim, Byung Chul

AU - Kim, Yong Sun

AU - Kang, Il Jun

AU - Kumar, Chandra C.

N1 - Funding Information: This work was supported by grant from Hallym Academy of Science, Hallym University, 1996 (to I.J. Kang). We wish to thank Dr. Ron. Prywes (Columbia University, USA) for providing us with SRE-luciferase plasmids. We also wish to thank Dr. Allan Hall (University College, London, UK) for providing us pEXV-Rac plasmid.

PY - 1997/4/7

Y1 - 1997/4/7

N2 - In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.

AB - In the present study, we investigated whether hydrogen peroxide activates c-fos serum response element (SRE) in Rat-2 fibroblast cells. By transient transfection analysis, exogenous H2O2 stimulated SRE-dependent reporter gene activity in a dose and time-dependent manner. Also, we examined the role of Rac GTPase and phospholipase A2 (PLA2) in the H2O2-induced SRE activation. Either transfection of a dominant negative Rac mutant, RacN17, plasmid or pretreatment of mepacrine, a potent inhibitor of PLA2, blocked H2O2-induced SRE activation dramatically. Together, these findings suggest a critical role of 'Rac and subsequent activation of phospholipase A2' in the signaling pathway of H2O2 to SRE.

KW - Arachidonic acid

KW - Hydrogen peroxide

KW - Phospholipase A

KW - Rac GTPase

KW - Serum response element

KW - c-fos

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Essential role of Rac GTPase in hydrogen peroxide-induced activation of c-fos serum response element

Abstract

Keywords

ASJC Scopus subject areas

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