EST analysis of functional genes associated with cell wall biosynthesis and modification in the secondary xylem of the yellow poplar (Liriodendron tulipifera) stem during early stage of tension wood formation

Hyunjung Jin, Jihye Do, Dahyun Moon, Eun Woon Noh, Wook Kim, Mi Kwon

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A cDNA library was constructed from secondary xylem in the stem of a 2-year-old yellow poplar after being bent for 6 h with a 45° configuration to isolate genes related to cell wall modification during the early stages of tension wood formation. A total of 6,141 ESTs were sequenced to generate a database of 5,982 high-quality expressed sequence tags (ESTs). These sequences were clustered into 1,733 unigenes, including 822 contigs and 911 singletons. Homologs of the genes regulate many aspects of secondary xylem development, including those for primary and secondary metabolism, plant growth hormones, transcription factors, cell wall biosynthesis and modification, and stress responses. Although there were only 1,733 annotated ESTs (28. 9%), the annotated ESTs obtained in this study provided sequences for a broad array of transcripts expressed in the stem upon mechanical bending, and the majority of them were the first representatives of their respective gene families in Liriodendron tulipifera. In the case of lignin, xylem-specific COMTs were identified and their expressions were significantly downregulated in the tension wood-forming tissues. Additionally, the majority of the auxin- and BR-related genes were downregulated significantly in response to mechanical bending treatment. Despite the small number of ESTs sequenced in this study, many genes that are relevant to cell wall biosynthesis and modification have been isolated. Expression analysis of selected genes allow us to identify the regulatory genes that may perform essential functions during the early stages of tension wood formation and associated cell wall modification.

Original languageEnglish
Pages (from-to)959-977
Number of pages19
JournalPlanta
Volume234
Issue number5
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Liriodendron
tension wood
Xylem
secondary xylem
Liriodendron tulipifera
Expressed Sequence Tags
expressed sequence tags
Cell Wall
Sequence Analysis
cell walls
biosynthesis
stems
Genes
genes
Down-Regulation
Secondary Metabolism
unigenes
Indoleacetic Acids
Plant Growth Regulators
Lignin

Keywords

  • Cell wall
  • EST
  • Lignin
  • Liriodendron tulipifera
  • Tension wood

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

EST analysis of functional genes associated with cell wall biosynthesis and modification in the secondary xylem of the yellow poplar (Liriodendron tulipifera) stem during early stage of tension wood formation. / Jin, Hyunjung; Do, Jihye; Moon, Dahyun; Noh, Eun Woon; Kim, Wook; Kwon, Mi.

In: Planta, Vol. 234, No. 5, 01.11.2011, p. 959-977.

Research output: Contribution to journalArticle

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