Establishment and characterization of chromosomal aberrations in human cholangiocarcinoma cell lines by cross-species color banding

Dae Ghon Kim, Soo Yeun Park, Kyung Ran You, Gi Bong Lee, Hyun Kim, Woo Sung Moon, Yong Hyuck Chun, Sun-Hwa Park

Research output: Contribution to journalArticle

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Abstract

Cholangiocarcinoma (CC), a malignant neoplasm of the biliary epithelium, is usually fatal because of difficulty in early diagnosis and lack of availability of effective therapy. Furthermore, little is known about the genetics and biology of CC. Only a few reports concerning cytogenetic studies of CC have been published, and few cell lines have been established. We recently established four CC cell lines, designated as SCK, JCK, Cho-CK, and Choi-CK, and report the first application of cross-species color banding (RxFISH) and multiple chromosome painting for the characterization of the chromosomal rearrangements of these CC cell lines. Each cell line had unique modal karyotypic characteristics and showed a variable number of numerical and structural clonal cytogenetic aberrations. Chromosomes 3, 6, 7, 8, 12, 14, 17, and 18 were commonly involved in structural abnormalities. Homogeneously staining regions were determined in SCK and JCK, and double minute chromosomes were found in Cho-CK. The chromosomal aberrations of the four CC cell lines were effectively analyzed by RxFISH and FISH with multiple chromosome painting probes. The nonrandom rearrangements suggest candidate regions for isolation of genes related to CC. (C) 2001 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalGenes Chromosomes and Cancer
Volume30
Issue number1
DOIs
Publication statusPublished - 2001 Jan 1

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Cholangiocarcinoma
Chromosome Aberrations
Color
Cell Line
Chromosome Painting
Chromosomes, Human, Pair 6
Chromosomes, Human, Pair 3
Cytogenetics
Early Diagnosis
Epithelium
Chromosomes
Staining and Labeling
Genes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

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Establishment and characterization of chromosomal aberrations in human cholangiocarcinoma cell lines by cross-species color banding. / Kim, Dae Ghon; Park, Soo Yeun; You, Kyung Ran; Lee, Gi Bong; Kim, Hyun; Moon, Woo Sung; Chun, Yong Hyuck; Park, Sun-Hwa.

In: Genes Chromosomes and Cancer, Vol. 30, No. 1, 01.01.2001, p. 48-56.

Research output: Contribution to journalArticle

Kim, Dae Ghon ; Park, Soo Yeun ; You, Kyung Ran ; Lee, Gi Bong ; Kim, Hyun ; Moon, Woo Sung ; Chun, Yong Hyuck ; Park, Sun-Hwa. / Establishment and characterization of chromosomal aberrations in human cholangiocarcinoma cell lines by cross-species color banding. In: Genes Chromosomes and Cancer. 2001 ; Vol. 30, No. 1. pp. 48-56.
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