Establishment and characterization of three immortal bovine muscular epithelial cell lines

Xun Jin, Joong Seob Lee, Sungwook Kwak, Soo Yeon Lee, Ji Eun Jung, Tae Kyung Kim, Chenxiong Xu, Zhongshan Hong, Zhehu Li, Sun Myung Kim, Xumin Pian, Dong Hee Lee, Jong Taek Yoon, Seungkwon You, Yun Jaie Choi, Hyunggee Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack p16INK4a regulatory function, compared to primary BME cells that were growth arrested by enforced expression of p16INK4a. In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.

Original languageEnglish
Pages (from-to)29-33
Number of pages5
JournalMolecules and Cells
Volume21
Issue number1
Publication statusPublished - 2006 Feb 28

Fingerprint

Epithelial Cells
Cell Line
Keratins
HeLa Cells
Agar
Phenotype
Muscles
Growth
Population
Proteins

Keywords

  • Epithelial cell
  • Immortalization
  • Muscle
  • SV40LT
  • Telomerase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Jin, X., Lee, J. S., Kwak, S., Lee, S. Y., Jung, J. E., Kim, T. K., ... Kim, H. (2006). Establishment and characterization of three immortal bovine muscular epithelial cell lines. Molecules and Cells, 21(1), 29-33.

Establishment and characterization of three immortal bovine muscular epithelial cell lines. / Jin, Xun; Lee, Joong Seob; Kwak, Sungwook; Lee, Soo Yeon; Jung, Ji Eun; Kim, Tae Kyung; Xu, Chenxiong; Hong, Zhongshan; Li, Zhehu; Kim, Sun Myung; Pian, Xumin; Lee, Dong Hee; Yoon, Jong Taek; You, Seungkwon; Choi, Yun Jaie; Kim, Hyunggee.

In: Molecules and Cells, Vol. 21, No. 1, 28.02.2006, p. 29-33.

Research output: Contribution to journalArticle

Jin, X, Lee, JS, Kwak, S, Lee, SY, Jung, JE, Kim, TK, Xu, C, Hong, Z, Li, Z, Kim, SM, Pian, X, Lee, DH, Yoon, JT, You, S, Choi, YJ & Kim, H 2006, 'Establishment and characterization of three immortal bovine muscular epithelial cell lines', Molecules and Cells, vol. 21, no. 1, pp. 29-33.
Jin X, Lee JS, Kwak S, Lee SY, Jung JE, Kim TK et al. Establishment and characterization of three immortal bovine muscular epithelial cell lines. Molecules and Cells. 2006 Feb 28;21(1):29-33.
Jin, Xun ; Lee, Joong Seob ; Kwak, Sungwook ; Lee, Soo Yeon ; Jung, Ji Eun ; Kim, Tae Kyung ; Xu, Chenxiong ; Hong, Zhongshan ; Li, Zhehu ; Kim, Sun Myung ; Pian, Xumin ; Lee, Dong Hee ; Yoon, Jong Taek ; You, Seungkwon ; Choi, Yun Jaie ; Kim, Hyunggee. / Establishment and characterization of three immortal bovine muscular epithelial cell lines. In: Molecules and Cells. 2006 ; Vol. 21, No. 1. pp. 29-33.
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