Establishment and characterization of three immortal bovine muscular epithelial cell lines

Xun Jin, Joong Seob Lee, Sungwook Kwak, Soo Yeon Lee, Ji Eun Jung, Tae Kyung Kim, Chenxiong Xu, Zhongshan Hong, Zhehu Li, Sun Myung Kim, Xumin Pian, Dong Hee Lee, Jong Taek Yoon, Seungkwon You, Yun Jaie Choi, Hyunggee Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack p16INK4a regulatory function, compared to primary BME cells that were growth arrested by enforced expression of p16INK4a. In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.

Original languageEnglish
Pages (from-to)29-33
Number of pages5
JournalMolecules and cells
Volume21
Issue number1
Publication statusPublished - 2006 Feb 28

Keywords

  • Epithelial cell
  • Immortalization
  • Muscle
  • SV40LT
  • Telomerase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Establishment and characterization of three immortal bovine muscular epithelial cell lines'. Together they form a unique fingerprint.

  • Cite this

    Jin, X., Lee, J. S., Kwak, S., Lee, S. Y., Jung, J. E., Kim, T. K., Xu, C., Hong, Z., Li, Z., Kim, S. M., Pian, X., Lee, D. H., Yoon, J. T., You, S., Choi, Y. J., & Kim, H. (2006). Establishment and characterization of three immortal bovine muscular epithelial cell lines. Molecules and cells, 21(1), 29-33.