Establishment of a new strategy against Microcystis bloom using newly isolated lytic and toxin-degrading bacteria

Changsu Lee, Min Seo Jeon, Thi Thao Vo, Chulhwan Park, Jong Soon Choi, Joseph Kwon, Seong Woon Roh, Yoon-E Choi

Research output: Contribution to journalArticle

Abstract

Unwanted, rapid increases in the algal populations of water systems cause harmful algal blooms, which have recently become a major environmental problem. The cyanobacterium Microcystis aeruginosa is the most prevalent bloom species and is responsible for the majority of blooms in freshwater environments. In this study, we attempted to develop an eco-friendly method to suppress M. aeruginosa bloom based on a biological control using bacteria newly isolated from the soil. In a screen for bacteria with strong lethal activity toward Microcystis, we isolated Bacillus sp. T4 and characterised its algicidal activity. Microcystis aeruginosa cells were killed via indirect attack by compound(s) secreted by T4 bacteria. ELISA revealed a dramatic increase in extracellular microcystins in M. aeruginosa cultures upon treatment with T4. Therefore, we screened for bacteria that could degrade these toxins, and three new isolates (R12, S42 and S65) were identified. Simultaneous application of both T4 as a lytic agent and R12 or S42 as toxin-degrading bacteria could eliminate both Microcystis cells and its problematic toxin. Our eco-friendly approach, based on the application of newly isolated bacteria, provides a novel method to control harmful algal blooms.

Original languageEnglish
Pages (from-to)1795-1806
Number of pages12
JournalJournal of Applied Phycology
Volume30
Issue number3
DOIs
Publication statusPublished - 2018 Jun 1

Fingerprint

Microcystis
toxin
algal bloom
toxins
Microcystis aeruginosa
bacterium
bacteria
algal blooms
microcystins
freshwater environment
Bacillus (bacteria)
biological control
Cyanobacteria
cyanobacterium
enzyme-linked immunosorbent assay
cells
methodology
soil
water

Keywords

  • Algal bloom
  • Bacterial isolation
  • Biological control
  • Cyanobacterium
  • Microcystin degradation
  • Microcystis

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Establishment of a new strategy against Microcystis bloom using newly isolated lytic and toxin-degrading bacteria. / Lee, Changsu; Jeon, Min Seo; Vo, Thi Thao; Park, Chulhwan; Choi, Jong Soon; Kwon, Joseph; Roh, Seong Woon; Choi, Yoon-E.

In: Journal of Applied Phycology, Vol. 30, No. 3, 01.06.2018, p. 1795-1806.

Research output: Contribution to journalArticle

Lee, Changsu ; Jeon, Min Seo ; Vo, Thi Thao ; Park, Chulhwan ; Choi, Jong Soon ; Kwon, Joseph ; Roh, Seong Woon ; Choi, Yoon-E. / Establishment of a new strategy against Microcystis bloom using newly isolated lytic and toxin-degrading bacteria. In: Journal of Applied Phycology. 2018 ; Vol. 30, No. 3. pp. 1795-1806.
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