Establishment of an experimental system for intraperitoneal chemotherapy in a rat model

SUNWOO PARK, SOO JIN PARK, HEE SU LEE, JIYEON HAM, EUN JI LEE, JUNSIK KIM, SOOMIN RYU, AERAN SEOL, WHASUN LIM, JUNG CHAN LEE, GWONHWA SONG, HEE SEUNG KIM

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To establish an experimental system for comparing different methods of intraperitoneal chemotherapy in a rat model. Materials and Methods: We used six-week-old Sprague-Dawley rats, and created an early postoperative intraperitoneal chemotherapy (EPIC) system using 18-gauge syringes and evacuators, and a hyperthermic intraperitoneal chemotherapy (HIPEC) system using two peristaltic pumps which controlled the flow rate and temperature. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was achieved using a nozzle for dispersing aerosols at a flow rate up to 41.5 ml/min. The distribution and intensity of 0.2% trypan blue dye was compared among three methods. Results: The distribution was limited and the intensity was weak after EPIC, and the dye stained moderately in gravity-dependent regions after HIPEC. On the other hand, the distribution was the most comprehensive, and the intensity was the greatest after PIPAC. Conclusion: This experimental system in a rat model may reflect the comparative effect among EPIC, HIPEC and PIPAC in humans.

Original languageEnglish
Pages (from-to)2703-2710
Number of pages8
JournalIn Vivo
Volume35
Issue number5
DOIs
Publication statusPublished - 2021 Oct

Keywords

  • Experimental system
  • Intraperitoneal chemotherapy
  • Rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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