Establishment of novel cell lines latently infected with human immunodeficiency virus 1

You Take Oh, Kyung Chang Kim, Kee Jong Hong, Hak Sung Lee, Dae Ho Jang, Joo Shil Lee, Sang-Yun Choi, Sung Soon Kim, Byeong Sun Choi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Many human immunodeficiency virus 1 (HIV-1) researchers focus on the developing new antireservoir therapy to eradicate HIV-1 provirus from the HIV-1-infected patients. HIV-1 provirus is the major obstacle for effective HIV-1 treatment because it integrates into the host genome and can produce a virus progeny after stopping highly active antiretroviral therapy (HAART). We established two novel cell lines latently infected with HIV-1 by limiting dilution cloning of A3.01 cells infected with HIV-1. Analysis of the flanking sequence of HIV-1 proviral DNA integrated into chromosomal cellular DNA revealed that proviral DNA was inserted into different sites of different chromosomes in the two examined cell lines. In these lines, virus reactivation could be induced by a phorbol 12-myristate 13-acetate (PMA) treatment that resulted in a marked increase of the production HIV-1 p24 antigen and appearance of the infectious virus. The novel cell lines latently infected with HIV-1 represent further tool for the study of molecular mechanisms of viral latency and development of anti-reservoir therapy of HIV-1 infection.

Original languageEnglish
Pages (from-to)155-159
Number of pages5
JournalActa Virologica
Volume55
Issue number2
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

HIV-1
Cell Line
Proviruses
Viruses
DNA
Virus Latency
Highly Active Antiretroviral Therapy
Virus Diseases
Therapeutics
Sequence Analysis
Organism Cloning
Acetates
Chromosomes
Research Personnel
Genome
Antigens

Keywords

  • HIV integration
  • HIV-1 provirus
  • Latency
  • NCHA cell lines
  • Reactivation

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Oh, Y. T., Kim, K. C., Hong, K. J., Lee, H. S., Jang, D. H., Lee, J. S., ... Choi, B. S. (2011). Establishment of novel cell lines latently infected with human immunodeficiency virus 1. Acta Virologica, 55(2), 155-159. https://doi.org/10.4149/av_2011_02_155

Establishment of novel cell lines latently infected with human immunodeficiency virus 1. / Oh, You Take; Kim, Kyung Chang; Hong, Kee Jong; Lee, Hak Sung; Jang, Dae Ho; Lee, Joo Shil; Choi, Sang-Yun; Kim, Sung Soon; Choi, Byeong Sun.

In: Acta Virologica, Vol. 55, No. 2, 01.12.2011, p. 155-159.

Research output: Contribution to journalArticle

Oh, YT, Kim, KC, Hong, KJ, Lee, HS, Jang, DH, Lee, JS, Choi, S-Y, Kim, SS & Choi, BS 2011, 'Establishment of novel cell lines latently infected with human immunodeficiency virus 1', Acta Virologica, vol. 55, no. 2, pp. 155-159. https://doi.org/10.4149/av_2011_02_155
Oh, You Take ; Kim, Kyung Chang ; Hong, Kee Jong ; Lee, Hak Sung ; Jang, Dae Ho ; Lee, Joo Shil ; Choi, Sang-Yun ; Kim, Sung Soon ; Choi, Byeong Sun. / Establishment of novel cell lines latently infected with human immunodeficiency virus 1. In: Acta Virologica. 2011 ; Vol. 55, No. 2. pp. 155-159.
@article{8ace5ee5c286418786a03f9330a36e0a,
title = "Establishment of novel cell lines latently infected with human immunodeficiency virus 1",
abstract = "Many human immunodeficiency virus 1 (HIV-1) researchers focus on the developing new antireservoir therapy to eradicate HIV-1 provirus from the HIV-1-infected patients. HIV-1 provirus is the major obstacle for effective HIV-1 treatment because it integrates into the host genome and can produce a virus progeny after stopping highly active antiretroviral therapy (HAART). We established two novel cell lines latently infected with HIV-1 by limiting dilution cloning of A3.01 cells infected with HIV-1. Analysis of the flanking sequence of HIV-1 proviral DNA integrated into chromosomal cellular DNA revealed that proviral DNA was inserted into different sites of different chromosomes in the two examined cell lines. In these lines, virus reactivation could be induced by a phorbol 12-myristate 13-acetate (PMA) treatment that resulted in a marked increase of the production HIV-1 p24 antigen and appearance of the infectious virus. The novel cell lines latently infected with HIV-1 represent further tool for the study of molecular mechanisms of viral latency and development of anti-reservoir therapy of HIV-1 infection.",
keywords = "HIV integration, HIV-1 provirus, Latency, NCHA cell lines, Reactivation",
author = "Oh, {You Take} and Kim, {Kyung Chang} and Hong, {Kee Jong} and Lee, {Hak Sung} and Jang, {Dae Ho} and Lee, {Joo Shil} and Sang-Yun Choi and Kim, {Sung Soon} and Choi, {Byeong Sun}",
year = "2011",
month = "12",
day = "1",
doi = "10.4149/av_2011_02_155",
language = "English",
volume = "55",
pages = "155--159",
journal = "Acta Virologica",
issn = "0001-723X",
publisher = "AEP - Academic Electronic Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Establishment of novel cell lines latently infected with human immunodeficiency virus 1

AU - Oh, You Take

AU - Kim, Kyung Chang

AU - Hong, Kee Jong

AU - Lee, Hak Sung

AU - Jang, Dae Ho

AU - Lee, Joo Shil

AU - Choi, Sang-Yun

AU - Kim, Sung Soon

AU - Choi, Byeong Sun

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Many human immunodeficiency virus 1 (HIV-1) researchers focus on the developing new antireservoir therapy to eradicate HIV-1 provirus from the HIV-1-infected patients. HIV-1 provirus is the major obstacle for effective HIV-1 treatment because it integrates into the host genome and can produce a virus progeny after stopping highly active antiretroviral therapy (HAART). We established two novel cell lines latently infected with HIV-1 by limiting dilution cloning of A3.01 cells infected with HIV-1. Analysis of the flanking sequence of HIV-1 proviral DNA integrated into chromosomal cellular DNA revealed that proviral DNA was inserted into different sites of different chromosomes in the two examined cell lines. In these lines, virus reactivation could be induced by a phorbol 12-myristate 13-acetate (PMA) treatment that resulted in a marked increase of the production HIV-1 p24 antigen and appearance of the infectious virus. The novel cell lines latently infected with HIV-1 represent further tool for the study of molecular mechanisms of viral latency and development of anti-reservoir therapy of HIV-1 infection.

AB - Many human immunodeficiency virus 1 (HIV-1) researchers focus on the developing new antireservoir therapy to eradicate HIV-1 provirus from the HIV-1-infected patients. HIV-1 provirus is the major obstacle for effective HIV-1 treatment because it integrates into the host genome and can produce a virus progeny after stopping highly active antiretroviral therapy (HAART). We established two novel cell lines latently infected with HIV-1 by limiting dilution cloning of A3.01 cells infected with HIV-1. Analysis of the flanking sequence of HIV-1 proviral DNA integrated into chromosomal cellular DNA revealed that proviral DNA was inserted into different sites of different chromosomes in the two examined cell lines. In these lines, virus reactivation could be induced by a phorbol 12-myristate 13-acetate (PMA) treatment that resulted in a marked increase of the production HIV-1 p24 antigen and appearance of the infectious virus. The novel cell lines latently infected with HIV-1 represent further tool for the study of molecular mechanisms of viral latency and development of anti-reservoir therapy of HIV-1 infection.

KW - HIV integration

KW - HIV-1 provirus

KW - Latency

KW - NCHA cell lines

KW - Reactivation

UR - http://www.scopus.com/inward/record.url?scp=80052793248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052793248&partnerID=8YFLogxK

U2 - 10.4149/av_2011_02_155

DO - 10.4149/av_2011_02_155

M3 - Article

VL - 55

SP - 155

EP - 159

JO - Acta Virologica

JF - Acta Virologica

SN - 0001-723X

IS - 2

ER -