Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23)

Sanghyun Park, Jeong Eun Yoo, Dongjin R. Lee, Jiho Jang, Myung Soo Cho, Dae-Sung Kim, Dong Wook Kim

Research output: Contribution to journalArticle

Abstract

Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry hESCs maintained pluripotency marker expression and exhibited the capacity to generate all 3 germ layers and a normal karyotype. After differentiation into mDA neurons, most PITX3 immunoreactivity overlapped with the red fluorescence of mCherry. This reporter cell line may be used to study the development of mDA neurons or to enrich mDA populations for transplantation.

Original languageEnglish
Article number101499
JournalStem Cell Research
Volume39
DOIs
Publication statusPublished - 2019 Aug 1

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Mesencephalon
Dopaminergic Neurons
Cell Line
Clustered Regularly Interspaced Short Palindromic Repeats
Germ Layers
Karyotype
Transcription Factors
Transplantation
Fluorescence
Maintenance
Human Embryonic Stem Cells
Population

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23). / Park, Sanghyun; Yoo, Jeong Eun; Lee, Dongjin R.; Jang, Jiho; Cho, Myung Soo; Kim, Dae-Sung; Kim, Dong Wook.

In: Stem Cell Research, Vol. 39, 101499, 01.08.2019.

Research output: Contribution to journalArticle

Park, Sanghyun ; Yoo, Jeong Eun ; Lee, Dongjin R. ; Jang, Jiho ; Cho, Myung Soo ; Kim, Dae-Sung ; Kim, Dong Wook. / Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23). In: Stem Cell Research. 2019 ; Vol. 39.
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