Ethanol decreases basal insulin secretion from HIT-T15 cells

Jun Seop Shin, Jae Jeong Lee, Jae Won Yang, Chan Wha Kim

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12 Citations (Scopus)

Abstract

Various epidemiological studies suggest that alcohol intake is one of the risk factors leading to type II or non-insulin-dependent diabetes mellitus (NIDDM), but the effect of alcohol on β-cell function remains unexplored. To study the mechanism of the diabetogenic action of ethanol, we investigated the effect of ethanol on β-cell functions using a single clonal β-cell line, HIT-T15 cells. When HIT cells were treated with ethanol, the metabolic activity judged by MTT assay was inhibited in dose- and time dependent manners, but cytotoxicity was not observed. Ethanol also inhibited basal insulin secretion by 30% compared to the untreated control. However, glucose-stimulated insulin secretion was not impaired by ethanol although the basal insulin secretion was inhibited. These results imply that ethanol exert β-cells to overwork in order to compensate inhibition of the basal secretion. This finding may at least in part explain the diabetogenic action of ethanol.

Original languageEnglish
Pages (from-to)1989-1997
Number of pages9
JournalLife Sciences
Volume70
Issue number17
DOIs
Publication statusPublished - 2002 Mar 15

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Keywords

  • Alcohol
  • Insulin secretion
  • NIDDM

ASJC Scopus subject areas

  • Pharmacology

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