Ethanol-dispersed polymer nanofibers as a highly selective cell isolation and release platform for CD4 + T lymphocytes

Seung Hyun Jun, Kwanghee Kim, Hyo Jin An, Byoung Chan Kim, Chung Hee Sonn, Miju Kim, Junsang Doh, Cassian Yee, Kyung Mi Lee, Jungbae Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4 + T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4 + T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4 + T cells for the purpose of cell therapies.

Original languageEnglish
Pages (from-to)4448-4455
Number of pages8
JournalAdvanced Functional Materials
Volume22
Issue number21
DOIs
Publication statusPublished - 2012 Nov 7

Keywords

  • CD4 T lymphocyte
  • cell separation
  • cell transplantation
  • electrospun polymer nanofibers
  • ethanol dispersion

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Fingerprint Dive into the research topics of 'Ethanol-dispersed polymer nanofibers as a highly selective cell isolation and release platform for CD4 <sup>+</sup> T lymphocytes'. Together they form a unique fingerprint.

  • Cite this