Ethanol-dispersed polymer nanofibers as a highly selective cell isolation and release platform for CD4 + T lymphocytes

Seung Hyun Jun, Kwanghee Kim, Hyo Jin An, Byoung Chan Kim, Chung Hee Sonn, Miju Kim, Junsang Doh, Cassian Yee, Kyung-Mi Lee, Jungbae Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A novel cell isolation and release platform using electrospun polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers is presented. Ethanol treatment of PS-PSMA nanofibers, employed for the purpose of sterilization, significantly increases their inter-fiber space for both antibody conjugation and subsequent cell separation. For the selective isolation of CD4 + T cells from heterogeneous mixtures of mouse lymph nodes, capture efficiencies of up to 100% are achieved while maintaining cellular integrity and viability. Once captured, CD4 + T lymphocytes can also be released from the NF scaffolds, further demonstrating its potential functionality as an immune cell-supporting and releasing matrix. This is the first demonstration of lymphocyte-culture scaffolds enabling selective isolation, accommodation, and sustained release of CD4 + T cells for the purpose of cell therapies.

Original languageEnglish
Pages (from-to)4448-4455
Number of pages8
JournalAdvanced Functional Materials
Volume22
Issue number21
DOIs
Publication statusPublished - 2012 Nov 7

Fingerprint

T-cells
lymphocytes
Nanofibers
isolation
polystyrene
Polymers
Ethanol
ethyl alcohol
platforms
Polystyrenes
Maleic anhydride
Styrene
polymers
anhydrides
cells
Lymphocytes
Scaffolds (biology)
Cell culture
Antibodies
Scaffolds

Keywords

  • CD4 T lymphocyte
  • cell separation
  • cell transplantation
  • electrospun polymer nanofibers
  • ethanol dispersion

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Ethanol-dispersed polymer nanofibers as a highly selective cell isolation and release platform for CD4 + T lymphocytes. / Jun, Seung Hyun; Kim, Kwanghee; An, Hyo Jin; Kim, Byoung Chan; Sonn, Chung Hee; Kim, Miju; Doh, Junsang; Yee, Cassian; Lee, Kyung-Mi; Kim, Jungbae.

In: Advanced Functional Materials, Vol. 22, No. 21, 07.11.2012, p. 4448-4455.

Research output: Contribution to journalArticle

Jun, Seung Hyun ; Kim, Kwanghee ; An, Hyo Jin ; Kim, Byoung Chan ; Sonn, Chung Hee ; Kim, Miju ; Doh, Junsang ; Yee, Cassian ; Lee, Kyung-Mi ; Kim, Jungbae. / Ethanol-dispersed polymer nanofibers as a highly selective cell isolation and release platform for CD4 + T lymphocytes. In: Advanced Functional Materials. 2012 ; Vol. 22, No. 21. pp. 4448-4455.
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