Ethanol production from rice straw using optimized aqueous-ammonia soaking pretreatment and simultaneous saccharification and fermentation processes

Ja Kyong Ko, Jin Seop Bak, Min Woo Jung, Hee Jin Lee, In-Geol Choi, Tae Hyun Kim, Kyoung Heon Kim

Research output: Contribution to journalArticle

220 Citations (Scopus)


Rice straw was pretreated using aqueous-ammonia solution at moderate temperatures to enable production of the maximum amount of fermentable sugars from enzymatic hydrolysis. The effects of various operating variables including pretreatment temperature, pretreatment time, the concentration of ammonia and the solid-to-liquid ratio on the degree of lignin removal and the enzymatic digestibility were optimized using response surface methodology. The optimal reaction conditions, which resulted in an enzymatic digestibility of 71.1%, were found to be 69 °C, 10 h and an ammonia concentration of 21% (w/w). The effects of different commercial cellulases and the additional effect of a non-cellulolytic enzyme, xylanase, were also evaluated. Additionally, simultaneous saccharification and fermentation was conducted with rice straw to assess the ethanol production yield and productivity.

Original languageEnglish
Pages (from-to)4374-4380
Number of pages7
JournalBioresource Technology
Issue number19
Publication statusPublished - 2009 Oct 1



  • Ammonia
  • Ethanol
  • Lignin
  • Lignocellulose
  • Pretreatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this