EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas

Jae Min Lee; Hyuk Soon Choi; Hoon Jai Chun; Eun Sun Kim; Bora Keum; Yeon Seok Seo; Yoon Tae Jeen; Hong Sik Lee; Soon Ho Um; Chang Duck Kim; Hong Bae Kim

doi:10.1007/s00464-018-6425-4

EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas

Jae Min Lee, Hyuk Soon Choi, Hoon Jai Chun, Eun Sun Kim, Bora Keum, Yeon Seok Seo, Yoon Tae Jeen, Hong Sik Lee, Soon Ho Um, Chang Duck Kim, Hong Bae Kim

* New professors

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.

Original language	English
Pages (from-to)	658-662
Number of pages	5
Journal	Surgical Endoscopy
Volume	33
Issue number	2
DOIs	https://doi.org/10.1007/s00464-018-6425-4
Publication status	Published - 2019 Feb 15

Keywords

Animal
EUS
Endoscopy
Irreversible electroporation
Pancreas

ASJC Scopus subject areas

Surgery

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10.1007/s00464-018-6425-4

Cite this

@article{a26ff038a5a144c5b6b6e6b826b13940,

title = "EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas",

abstract = "Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.",

keywords = "Animal, EUS, Endoscopy, Irreversible electroporation, Pancreas",

author = "Lee, {Jae Min} and Choi, {Hyuk Soon} and Chun, {Hoon Jai} and Kim, {Eun Sun} and Bora Keum and Seo, {Yeon Seok} and Jeen, {Yoon Tae} and Lee, {Hong Sik} and Um, {Soon Ho} and Kim, {Chang Duck} and Kim, {Hong Bae}",

note = "Funding Information: Fig. 4 Immunohistopathology with TUNEL assay in the pancreas parenchyma. Round ablated zone with apoptosis and visualized pyknosis and karyorrhexis (TUNEL, ×100) Acknowledgements This study was supported by a College of Medicine, Korea University Grant (No. K1523601) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2018R1C1B6006044). Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2019",

month = feb,

day = "15",

doi = "10.1007/s00464-018-6425-4",

language = "English",

volume = "33",

pages = "658--662",

journal = "Surgical Endoscopy",

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TY - JOUR

T1 - EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas

AU - Lee, Jae Min

AU - Choi, Hyuk Soon

AU - Chun, Hoon Jai

AU - Kim, Eun Sun

AU - Keum, Bora

AU - Seo, Yeon Seok

AU - Jeen, Yoon Tae

AU - Lee, Hong Sik

AU - Um, Soon Ho

AU - Kim, Chang Duck

AU - Kim, Hong Bae

N1 - Funding Information: Fig. 4 Immunohistopathology with TUNEL assay in the pancreas parenchyma. Round ablated zone with apoptosis and visualized pyknosis and karyorrhexis (TUNEL, ×100) Acknowledgements This study was supported by a College of Medicine, Korea University Grant (No. K1523601) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2018R1C1B6006044). Publisher Copyright: © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2019/2/15

Y1 - 2019/2/15

N2 - Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.

AB - Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.

KW - Animal

KW - EUS

KW - Endoscopy

KW - Irreversible electroporation

KW - Pancreas

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SN - 0930-2794

VL - 33

SP - 658

EP - 662

JO - Surgical Endoscopy

JF - Surgical Endoscopy

IS - 2

ER -

EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas

Abstract

Keywords

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