EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas

Jae Min Lee, Hyuk Soon Choi, Hoon-Jai Chun, Eun-Sun Kim, Bora Keum, Yeon Seok Seo, Yoon Tae Jeen, Hong Sik Lee, Soon-Ho Um, Chang Duck Kim, Hong Bae Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.

Original languageEnglish
JournalSurgical Endoscopy
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Keywords

  • Animal
  • Endoscopy
  • EUS
  • Irreversible electroporation
  • Pancreas

ASJC Scopus subject areas

  • Surgery

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