EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.

Original languageEnglish
JournalSurgical Endoscopy
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Electroporation
Needles
Pancreas
Electrodes
Swine
In Situ Nick-End Labeling
Punctures
Cell Death
Immunohistochemistry
Staining and Labeling
Hemorrhage
Equipment and Supplies

Keywords

  • Animal
  • Endoscopy
  • EUS
  • Irreversible electroporation
  • Pancreas

ASJC Scopus subject areas

  • Surgery

Cite this

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title = "EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas",
abstract = "Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.",
keywords = "Animal, Endoscopy, EUS, Irreversible electroporation, Pancreas",
author = "Lee, {Jae Min} and Choi, {Hyuk Soon} and Hoon-Jai Chun and Eun-Sun Kim and Bora Keum and Seo, {Yeon Seok} and Jeen, {Yoon Tae} and Lee, {Hong Sik} and Soon-Ho Um and Kim, {Chang Duck} and Kim, {Hong Bae}",
year = "2018",
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doi = "10.1007/s00464-018-6425-4",
language = "English",
journal = "Surgical Endoscopy",
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T1 - EUS-guided irreversible electroporation using endoscopic needle-electrode in porcine pancreas

AU - Lee, Jae Min

AU - Choi, Hyuk Soon

AU - Chun, Hoon-Jai

AU - Kim, Eun-Sun

AU - Keum, Bora

AU - Seo, Yeon Seok

AU - Jeen, Yoon Tae

AU - Lee, Hong Sik

AU - Um, Soon-Ho

AU - Kim, Chang Duck

AU - Kim, Hong Bae

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.

AB - Background: Endoscopic irreversible electroporation (IRE) can be performed using a flexible, thin, needle-shaped electrode for an endoscopic ultrasound (EUS)-guided procedure. This study aimed to evaluate the feasibility and efficacy of performing EUS-guided IRE with endoscopic needle-electrode in porcine pancreas. Methods: Experimental endoscopic IRE on the pancreas were performed by EUS-guided approach in three pigs and compared with surgical approach in three pigs. The animals were killed after 24 h and their pancreases collected. Results: IRE ablation using endoscopic needle-electrode was successful technically in EUS-guided approaches for the pancreas. Immediately following IRE, the ablated pancreatic tissue showed no gross change except focal hemorrhage. H&E staining presented a well-demarcated ablation site measuring 1.0–1.5 cm in diameter in the pancreas. TUNEL immunohistochemistry showed diffuse cell death along the puncture site 24 h after IRE. No complication was observed in pigs after endoscopic IRE ablation. Conclusion: EUS-guided IRE ablation was feasible and effective for pancreas using the newly developed device.

KW - Animal

KW - Endoscopy

KW - EUS

KW - Irreversible electroporation

KW - Pancreas

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