Evaluation of a high throughput toxicity biosensor and comparison with a Daphnia magna bioassay

Byoung Chan Kim, Kyeong Seo Park, Sang Don Kim, Man Bock Gu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A high throughput toxicity biosensor has been designed and constructed using recombinant Escherichia coli cells, containing stress specific promoters (recA, fabA, or katG) or constitutive promoters (lac) fused to luciferase genes originating from Vibrio fisheri. These genetically engineered cells were immobilized in 96 well plates. By optimizing cell immobilization conditions and the strains' response specificity to toxic chemicals, bioluminescent outputs decreased or increased dose-dependently upon adding test chemicals. However, to date the toxicity data obtained using this biosensor have not been compared with the results of other toxicity tests. Phenolics were chosen to evaluate the correlation between the LD50 and the EC50 (GC2) or EC120 (DPD2540) of Daphnia magna and E. coli, respectively. Toxicity data obtained from constitutive strains by bioluminescent level decrements were compared with the results from D. magna as a standard. LD50 values were used as parameters of D. magna toxicity and EC50 of EC120 values were used for the immobilized biosensor. In the DPD2540 test, phenolics, membrane damaging toxic chemicals, for testing immobilized stress specific bacterial strains trigger dose-dependant bioluminescence increase within specific concentration. Although the stress specific responsiveness from the strains could not be compared with D. magna's LD50 values, these responses offer additional information, such as upon the mode of toxic action in the sample, in addition to the cellular toxicity results as indicated by the EC50. This novel high throughput toxicity biosensor can be implemented to investigate the toxicity of any other soluble materials, and can be used as a standardization tool for the evaluation of toxicity.

Original languageEnglish
Pages (from-to)821-826
Number of pages6
JournalBiosensors and Bioelectronics
Volume18
Issue number5-6
DOIs
Publication statusPublished - 2003 May 1
Externally publishedYes

Fingerprint

Daphnia
Bioassay
Biosensing Techniques
Biosensors
Biological Assay
Toxicity
Lethal Dose 50
Throughput
Poisons
Escherichia coli
Toxic Actions
Toxicity Tests
Immobilized Cells
Vibrio
Luciferases
Immobilization
Cell immobilization
Bioluminescence
Membranes
Genes

Keywords

  • Bioluminescence
  • Daphnia magna
  • High throughput biosensor
  • Toxicity

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Evaluation of a high throughput toxicity biosensor and comparison with a Daphnia magna bioassay. / Kim, Byoung Chan; Park, Kyeong Seo; Kim, Sang Don; Gu, Man Bock.

In: Biosensors and Bioelectronics, Vol. 18, No. 5-6, 01.05.2003, p. 821-826.

Research output: Contribution to journalArticle

Kim, Byoung Chan ; Park, Kyeong Seo ; Kim, Sang Don ; Gu, Man Bock. / Evaluation of a high throughput toxicity biosensor and comparison with a Daphnia magna bioassay. In: Biosensors and Bioelectronics. 2003 ; Vol. 18, No. 5-6. pp. 821-826.
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