Evaluation of a malaria antibody enzyme immunoassay for use in blood screening

Jun Seo Oh, Su Kim Jang, Hwan Lee Chang, Hwa Nam Deok, Hyung Kim Sun, Dae Won Park, Chang Kyu Lee, Chae Seung Lim, Gil-Hong Park

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Transfusion-transmitted malaria is rare, but it may produce severe problem in the safety of blood transfusion due to the lack of reliable procedure to evaluate donors potentially exposed to malaria. Here, we evaluated a new enzyme-linked immunosorbent assay malaria antibody test (ELISA malaria antibody test, DiaMed, Switzerland) to detect antibodies to Plasmodium vivax (the indigenous malaria) in the blood samples in the Republic of Korea (ROK). Blood samples of four groups were obtained and analyzed; 100 samples from P.vivax infected patients, 35 from recovery patients, 366 from normal healthy individuals, and 325 from domestic travelers of non-endemic areas residents to risky areas of ROK. P.vivax antibody levels by ELISA were then compared to the results from microscopic examination and polymerase chain reaction (PCR) test. As a result, the ELISA malaria antibody test had a clinical sensitivity of 53.0% and a clinical specificity of 94.0% for P.vivax. Twenty out of 325 domestic travelers (6.2%) were reactive and 28 cases (8.6%) were doubtful. Of the reactive and doubtful cases, only two were confirmed as acute malaria by both microscopy and PCR test. Thus we found that the ELISA malaria antibody test was insufficiently sensitive for blood screening of P.vivax in ROK.

Original languageEnglish
Pages (from-to)75-78
Number of pages4
JournalMemorias do Instituto Oswaldo Cruz
Volume103
Issue number1
Publication statusPublished - 2008 Feb 1

Fingerprint

Immunoenzyme Techniques
Malaria
Screening
Blood
Enzyme-Linked Immunosorbent Assay
Republic of Korea
Antibodies
Enzymes
Polymerase chain reaction
Microscopic examination
Vivax Malaria
Polymerase Chain Reaction
Immunosorbents
Switzerland
Blood Transfusion
Microscopy
Assays
Tissue Donors
Safety
Recovery

Keywords

  • Antibody test
  • Malaria antibody
  • Plasmodium vivax
  • Transfusion

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology
  • Immunology
  • Microbiology (medical)
  • Clinical Biochemistry

Cite this

Evaluation of a malaria antibody enzyme immunoassay for use in blood screening. / Oh, Jun Seo; Jang, Su Kim; Chang, Hwan Lee; Deok, Hwa Nam; Sun, Hyung Kim; Park, Dae Won; Lee, Chang Kyu; Lim, Chae Seung; Park, Gil-Hong.

In: Memorias do Instituto Oswaldo Cruz, Vol. 103, No. 1, 01.02.2008, p. 75-78.

Research output: Contribution to journalArticle

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