Evaluation of a modified antimycobacterial susceptibility test using Middlebrook 7H10 agar containing 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride

Seung Heon Lee, D. H. Kong, S. H. Yun, K. R. Lee, K. P. Lee, S. G. Franzblau, E. Y. Lee, C. L. Chang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A rapid and accurate antimycobacterial susceptibility test is essential for effective treatment of tuberculosis. The aim of this study was to evaluate a modified method applying 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC) to the Clinical and Laboratory Standards Institute (CLSI) guideline for susceptibility testing of Mycobacterium tuberculosis. A total of 132 clinical isolates of M. tuberculosis, forty-eight isolates showing resistance to one or more of the first-line antituberculosis drugs, and eighty-four fully susceptible isolates were collected from hospitals of a nationwide distribution from June to September 2004. The modified procedure was conducted basically according to the agar-proportion method described in the CLSI Guideline both with STC 50 μg/mL. The amount of growth in each well was recorded and graded at 2nd and 3rd weeks after inoculation. After 3 weeks of incubation, the diagnostic sensitivity and specificity for the detection of drug-resistant strains of STC-containing agar proportion methods were 100%, except ethambutol-low level resistance, of which the diagnostic sensitivity was 93.4%. After two weeks of incubation in STC-containing agar proportion methods, one hundred of the 107 strain-drug combinations have shown drug resistance, indicating the sensitivity of 93.5%. Especially, all 41 isoniazid-resistant strains and 19 of 21 rifampin-resistant strains (90.5%) could be detected after two weeks of incubation. A modification of the agar proportion method using STC resulted in a reliable and more easily interpretable data, and detected most of resistant strains a week earlier than conventional method.

Original languageEnglish
Pages (from-to)548-551
Number of pages4
JournalJournal of Microbiological Methods
Volume66
Issue number3
DOIs
Publication statusPublished - 2006 Sep 1
Externally publishedYes

Fingerprint

Agar
Mycobacterium tuberculosis
Guidelines
Ethambutol
Isoniazid
Drug Combinations
Rifampin
2,3-diphenyl-5-thienyl-(2)-tetrazolium
Drug Resistance
Pharmaceutical Preparations
Tuberculosis
Sensitivity and Specificity
Growth

Keywords

  • Agar proportion method
  • Colorimetry
  • Drug resistance
  • Mycobacterium tuberculosis
  • Susceptibility test

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Cite this

Evaluation of a modified antimycobacterial susceptibility test using Middlebrook 7H10 agar containing 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride. / Lee, Seung Heon; Kong, D. H.; Yun, S. H.; Lee, K. R.; Lee, K. P.; Franzblau, S. G.; Lee, E. Y.; Chang, C. L.

In: Journal of Microbiological Methods, Vol. 66, No. 3, 01.09.2006, p. 548-551.

Research output: Contribution to journalArticle

Lee, Seung Heon ; Kong, D. H. ; Yun, S. H. ; Lee, K. R. ; Lee, K. P. ; Franzblau, S. G. ; Lee, E. Y. ; Chang, C. L. / Evaluation of a modified antimycobacterial susceptibility test using Middlebrook 7H10 agar containing 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride. In: Journal of Microbiological Methods. 2006 ; Vol. 66, No. 3. pp. 548-551.
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