TY - JOUR
T1 - Evaluation of a transgenic poplar as a potential biomass crop for biofuel production
AU - Jung, Young Hoon
AU - Cho, Hong Joo
AU - Lee, Jae Soon
AU - Noh, Eun Woon
AU - Park, Ohkmae K.
AU - Kim, Kyoung Heon
N1 - Funding Information:
This work was supported by grants from the Advanced Biomass R&D Center of Korea ( 2011-0031353 ) and the Pioneer Research Center Program (2011-0002327) funded by the Korean Government (MEST) . Grant support was also received from the Next-Generation BioGreen 21 Program ( PJ0080522011 and PJ008103032011 ) through the Rural Development Administration. Facility support at Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety is also acknowledged.
PY - 2013/2
Y1 - 2013/2
N2 - A transgenic poplar, in which the RabG3bCA gene from Arabidopsis was overexpressed, was analyzed for its biomass composition and enzymatic digestibility after chemical pretreatment. In comparison with a wild-type poplar (WT), the transgenic poplar (OX8) showed 9.8% higher glucan content. The levels of other biomass components did not differ greatly between WT and OX8. When WT and OX8 samples were pretreated by sulfuric acid (1%, w/v at 190 °C), sodium hydroxide (1%, w/v at 190 °C), or ammonia (14%, w/w at 80 °C), the washed pretreated solids of OX8 exhibited a higher enzymatic digestibility than those of WT in each chemical pretreatment. The sodium hydroxide pretreatment was the most effective among the three pretreatment processes, showing 58.7% and 69.4% of theoretical glucose yield from the saccharification of pretreated OX8 and WT, respectively. The transgenic poplar, growing faster and taller, was found to contain more glucan and have a higher enzymatic digestibility than WT.
AB - A transgenic poplar, in which the RabG3bCA gene from Arabidopsis was overexpressed, was analyzed for its biomass composition and enzymatic digestibility after chemical pretreatment. In comparison with a wild-type poplar (WT), the transgenic poplar (OX8) showed 9.8% higher glucan content. The levels of other biomass components did not differ greatly between WT and OX8. When WT and OX8 samples were pretreated by sulfuric acid (1%, w/v at 190 °C), sodium hydroxide (1%, w/v at 190 °C), or ammonia (14%, w/w at 80 °C), the washed pretreated solids of OX8 exhibited a higher enzymatic digestibility than those of WT in each chemical pretreatment. The sodium hydroxide pretreatment was the most effective among the three pretreatment processes, showing 58.7% and 69.4% of theoretical glucose yield from the saccharification of pretreated OX8 and WT, respectively. The transgenic poplar, growing faster and taller, was found to contain more glucan and have a higher enzymatic digestibility than WT.
KW - Biofuel
KW - Biomass
KW - Enzymatic digestibility
KW - Pretreatment
KW - Transgenic poplar
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U2 - 10.1016/j.biortech.2012.12.074
DO - 10.1016/j.biortech.2012.12.074
M3 - Article
C2 - 23298768
AN - SCOPUS:84873723254
VL - 129
SP - 639
EP - 641
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -
