Evaluation of antitumor metastasis via immunostimulating activities of pectic polysaccharides isolated from radish leaves

Seung U. Son, Ho Young Park, Hyung Joo Suh, Kwang Soon Shin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We investigated the antimetastatic effects of polysaccharides (RWP) isolated from radish leaves by hot water extraction and ethanol precipitation. From the sugar composition analysis, RWP was estimated to be a pectic polysaccharide. Peritoneal macrophages stimulated with RWP significantly augmented the production of various cytokines. In an assay for natural killer (NK) cell activity, both intravenous (i.v.) and oral administration of RWP significantly enhanced NK cells cytotoxicity against YAC-1 lymphoma. In an experiment on lung metastasis of Colon26-M3.1 carcinoma, prophylactic i.v. and oral administration of RWP inhibited metastasis by 77.2% and 55.0%, respectively, at 1000 μg/mouse. Furthermore, pretreatment with anti-asialo GM1 for NK cells depletion partly suppressed the inhibitory effect of RWP on lung metastasis. Thus, our study results suggest that RWP has superior antimetastatic activity, and this effect is related to the activation of various immune factors.

Original languageEnglish
Article number104639
JournalJournal of Functional Foods
Volume85
DOIs
Publication statusPublished - 2021 Oct

Keywords

  • Anti-metastasis
  • Macrophage activation
  • NK cell cytotoxicity
  • Radish leaf polysaccharide

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Fingerprint

Dive into the research topics of 'Evaluation of antitumor metastasis via immunostimulating activities of pectic polysaccharides isolated from radish leaves'. Together they form a unique fingerprint.

Cite this