Evaluation of Cell-Penetrating Peptides Using Microfluidic In Vitro 3D Brain Endothelial Barrier

Bohye Chung, Jaehoon Kim, Jiyoung Nam, Hyunho Kim, Yeju Jeong, Hui wen Liu, Youngkyu Cho, Yong Ho Kim, Hyun Jeong Oh, Seok Chung

Research output: Contribution to journalArticle

Abstract

In drug delivery to the human brain, blood vessels are a significant hurdle because they restrict the entry of most solutes to protect brain. To overcome this hurdle, an in vitro 3D model for brain endothelial barrier is developed using a microfluidic device with hydrogel providing a 3D extracellular matrix scaffold. Using the model, peptides known to utilize receptor-mediated transcytosis are verified, which has been one of the most promising mechanisms for brain-specific penetration. The cytotoxicity and cellular damage to the peptide are investigated and the receptor-mediated transcytosis and brain endothelial specific penetrating abilities of the peptides in a quantitative manner are demonstrated. As a preclinical test, applying the quantification assays conducted in this study are suggested, including the penetrating ability, cytotoxicity, endothelial damage, and receptor specificity. Using this microfluidic device as an in vitro platform for evaluating various brain targeting drugs and drug carrier candidates is also proposed.

Original languageEnglish
JournalMacromolecular Bioscience
DOIs
Publication statusAccepted/In press - 2020 Jan 1

Keywords

  • blood brain barrier
  • cell-penetrating peptide
  • receptor mediated transcytosis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Evaluation of Cell-Penetrating Peptides Using Microfluidic In Vitro 3D Brain Endothelial Barrier'. Together they form a unique fingerprint.

  • Cite this

    Chung, B., Kim, J., Nam, J., Kim, H., Jeong, Y., Liu, H. W., Cho, Y., Kim, Y. H., Oh, H. J., & Chung, S. (Accepted/In press). Evaluation of Cell-Penetrating Peptides Using Microfluidic In Vitro 3D Brain Endothelial Barrier. Macromolecular Bioscience. https://doi.org/10.1002/mabi.201900425