Evaluation of immuno- and reproductive toxicities and association between immunotoxicological and genotoxicological parameters in waste incineration workers

Eunha Oh, Eun Il Lee, Hosub Im, Hyung S. Kang, Woon W. Jung, Nam Hee Won, Eun M. Kim, Dong Geun Sul

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Abstract

In this study, we investigated immunotoxicity levels of 2,3,7,8-tetrachlorodibenzo-p-dioxin in 31 waste incineration workers and in 84 control subjects, and evaluated the association between immunological and genotoxicological parameters. DNA damage in mononuclear and polynuclear lymphocytes, and the level of the urinary metabolites, 1-OHP and 2-naphthol, were evaluated in both waste incineration workers and control subjects. Significant differences were detected in these values between exposed and control groups. Number of sperms was lower in the waste incineration workers than in the control subjects, as was the percentage of motile sperms, but a significant difference existed only in the number of sperms (p = 0.05). DNA damage in the spermatozoa of waste incineration workers and control subjects measured 1.40 ± 0.08 and 1.26 ± 0.03, respectively (p = 0.001). Specific surface antigens of peripheral lymphocytes, namely, CD3, CD4, CD8, CD19, and CD69 were used to measure immune status in waste incineration workers and control subjects. There was no significant difference in T- and B-cell profiles showed between waste incineration workers and control subjects (p = 0.684 and 0.157). In addition, the ratio of T helper cells to T cytotoxic cells was also not remarkably different between groups (p = 0.174). However, T-cell activation was found to be significantly higher in the waste incineration workers than in the control subjects (p = 0.001), although B-cell activation did not exhibit this trend. The levels of two cytokines (IL-4 an INF-γ) and four immunoglobulins (IgA, IgE, IgG, and IgM) were also measured in the experimental population. All immunoglobulin types were found in lower amounts in the waste incineration workers, but this diaparity was not significant one. In addition, the levels of two cytokines, IL-4 and INF-γ, were also found to be lower in the waste incineration workers than in the control subjects, and only in IL-4 was a significant difference determined to exist.

Original languageEnglish
Pages (from-to)65-80
Number of pages16
JournalToxicology
Volume210
Issue number1
DOIs
Publication statusPublished - 2005 May 15

Fingerprint

Incineration
Waste incineration
Toxicity
Association reactions
Interleukin-4
Sperm Count
Lymphocytes
DNA Damage
Spermatozoa
Immunoglobulins
B-Lymphocytes
Chemical activation
Cells
Cytokines
T-cells
DNA
Surface Antigens
Metabolites
Helper-Inducer T-Lymphocytes
Immunoglobulin E

Keywords

  • Comet assay
  • Cytokines
  • DNA damage
  • Immunoglobulin
  • Lymphocytes
  • PAHs spermatozoa
  • TCDD
  • Waste incineration

ASJC Scopus subject areas

  • Toxicology

Cite this

Evaluation of immuno- and reproductive toxicities and association between immunotoxicological and genotoxicological parameters in waste incineration workers. / Oh, Eunha; Lee, Eun Il; Im, Hosub; Kang, Hyung S.; Jung, Woon W.; Won, Nam Hee; Kim, Eun M.; Sul, Dong Geun.

In: Toxicology, Vol. 210, No. 1, 15.05.2005, p. 65-80.

Research output: Contribution to journalArticle

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