Evaluation of penetration of nanocarriers into red pepper leaf using confocal laser scanning microscopy

Minh Hiep Nguyen, Ji Sun Lee, In Cheon Hwang, Hyun Jin Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Penetration of nanocarriers into red pepper leaf was evaluated with the aim of identifying a novel way to enhance the efficacy of applied pesticides on the field. In addition, the effect of surface charge of the nanocarriers on penetration was also studied. Particularly, corn oil-nanoemulsions (NE) and chitosan coated NE (CH-NE) were successfully prepared with a high encapsulation efficiency, a high payload, a very small size, a small polydispersity index, and a high zeta potential. However, after being coated with chitosan, the zeta potential of NE changed from a negative charge to a positive charge. Penetration experiments were also carried out using a Franz diffusion cell followed by visualization using confocal laser scanning microscopy. The images of the vertical sections illustrated that penetration of nanocarriers (NE and CH-NE) into the red pepper leaf occurred very quickly. Nanocarriers fully penetrated the whole leaf after 60 min. Moreover, multi-depth images that paralleled the leaf surface (horizontal visualization) together with removal of plant-autofluorescent emissions and three-dimensional graphs describing the penetration of NE and CH-NE demonstrated that the negatively charged nanocarriers (NE) had a higher penetration rate compared to that of the positively charged nanocarriers (CH-NE).

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalCrop Protection
Volume66
DOIs
Publication statusPublished - 2014 Dec 1

Fingerprint

peppers
nanoemulsions
confocal laser scanning microscopy
chitosan
leaves
corn oil
encapsulation
nanocarriers
pesticides

Keywords

  • Chitosan
  • Deltamethrin
  • Leaf
  • Nanocarriers
  • Penetration
  • Zeta-potential

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Evaluation of penetration of nanocarriers into red pepper leaf using confocal laser scanning microscopy. / Nguyen, Minh Hiep; Lee, Ji Sun; Hwang, In Cheon; Park, Hyun Jin.

In: Crop Protection, Vol. 66, 01.12.2014, p. 61-66.

Research output: Contribution to journalArticle

@article{3f9ca702eeb540eeb330ba56e4926ad1,
title = "Evaluation of penetration of nanocarriers into red pepper leaf using confocal laser scanning microscopy",
abstract = "Penetration of nanocarriers into red pepper leaf was evaluated with the aim of identifying a novel way to enhance the efficacy of applied pesticides on the field. In addition, the effect of surface charge of the nanocarriers on penetration was also studied. Particularly, corn oil-nanoemulsions (NE) and chitosan coated NE (CH-NE) were successfully prepared with a high encapsulation efficiency, a high payload, a very small size, a small polydispersity index, and a high zeta potential. However, after being coated with chitosan, the zeta potential of NE changed from a negative charge to a positive charge. Penetration experiments were also carried out using a Franz diffusion cell followed by visualization using confocal laser scanning microscopy. The images of the vertical sections illustrated that penetration of nanocarriers (NE and CH-NE) into the red pepper leaf occurred very quickly. Nanocarriers fully penetrated the whole leaf after 60 min. Moreover, multi-depth images that paralleled the leaf surface (horizontal visualization) together with removal of plant-autofluorescent emissions and three-dimensional graphs describing the penetration of NE and CH-NE demonstrated that the negatively charged nanocarriers (NE) had a higher penetration rate compared to that of the positively charged nanocarriers (CH-NE).",
keywords = "Chitosan, Deltamethrin, Leaf, Nanocarriers, Penetration, Zeta-potential",
author = "Nguyen, {Minh Hiep} and Lee, {Ji Sun} and Hwang, {In Cheon} and Park, {Hyun Jin}",
year = "2014",
month = "12",
day = "1",
doi = "10.1016/j.cropro.2014.08.016",
language = "English",
volume = "66",
pages = "61--66",
journal = "Crop Protection",
issn = "0261-2194",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Evaluation of penetration of nanocarriers into red pepper leaf using confocal laser scanning microscopy

AU - Nguyen, Minh Hiep

AU - Lee, Ji Sun

AU - Hwang, In Cheon

AU - Park, Hyun Jin

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Penetration of nanocarriers into red pepper leaf was evaluated with the aim of identifying a novel way to enhance the efficacy of applied pesticides on the field. In addition, the effect of surface charge of the nanocarriers on penetration was also studied. Particularly, corn oil-nanoemulsions (NE) and chitosan coated NE (CH-NE) were successfully prepared with a high encapsulation efficiency, a high payload, a very small size, a small polydispersity index, and a high zeta potential. However, after being coated with chitosan, the zeta potential of NE changed from a negative charge to a positive charge. Penetration experiments were also carried out using a Franz diffusion cell followed by visualization using confocal laser scanning microscopy. The images of the vertical sections illustrated that penetration of nanocarriers (NE and CH-NE) into the red pepper leaf occurred very quickly. Nanocarriers fully penetrated the whole leaf after 60 min. Moreover, multi-depth images that paralleled the leaf surface (horizontal visualization) together with removal of plant-autofluorescent emissions and three-dimensional graphs describing the penetration of NE and CH-NE demonstrated that the negatively charged nanocarriers (NE) had a higher penetration rate compared to that of the positively charged nanocarriers (CH-NE).

AB - Penetration of nanocarriers into red pepper leaf was evaluated with the aim of identifying a novel way to enhance the efficacy of applied pesticides on the field. In addition, the effect of surface charge of the nanocarriers on penetration was also studied. Particularly, corn oil-nanoemulsions (NE) and chitosan coated NE (CH-NE) were successfully prepared with a high encapsulation efficiency, a high payload, a very small size, a small polydispersity index, and a high zeta potential. However, after being coated with chitosan, the zeta potential of NE changed from a negative charge to a positive charge. Penetration experiments were also carried out using a Franz diffusion cell followed by visualization using confocal laser scanning microscopy. The images of the vertical sections illustrated that penetration of nanocarriers (NE and CH-NE) into the red pepper leaf occurred very quickly. Nanocarriers fully penetrated the whole leaf after 60 min. Moreover, multi-depth images that paralleled the leaf surface (horizontal visualization) together with removal of plant-autofluorescent emissions and three-dimensional graphs describing the penetration of NE and CH-NE demonstrated that the negatively charged nanocarriers (NE) had a higher penetration rate compared to that of the positively charged nanocarriers (CH-NE).

KW - Chitosan

KW - Deltamethrin

KW - Leaf

KW - Nanocarriers

KW - Penetration

KW - Zeta-potential

UR - http://www.scopus.com/inward/record.url?scp=84907181870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907181870&partnerID=8YFLogxK

U2 - 10.1016/j.cropro.2014.08.016

DO - 10.1016/j.cropro.2014.08.016

M3 - Article

AN - SCOPUS:84907181870

VL - 66

SP - 61

EP - 66

JO - Crop Protection

JF - Crop Protection

SN - 0261-2194

ER -