Abstract
In the current study, the probiotic potential of approximately 350 strains of lactic acid bacteria isolated from Korean infant feces and Kimchi was investigated. Common probiotic properties of the bacterial strains, such as acid tolerance, bile tolerance and adhesion to human intestinal epithelial cells (HT-29 cells), were examined. Some strains were found to have immune modulatory and antimicrobial properties. Antagonistic activity against a panel of pathogenic bacteria was found to be strain dependent. To evaluate the immune modulatory activity of the strains, lymphocyte interferon (IFN)-γ secretion was determined in conjunction with cell proliferation. Some strains of Lactobacillus gasseri, L. fermentum and L. plantarum exhibited increased IFN-γ levels and lymphocyte proliferation. To evaluate the effects of these immune modulating lactobacilli on host life span, Caenorhabditis elegans was used as an in vivo model. Nematodes that were supplied heat-killed lactobacilli as a food source exhibited obvious differences in life span compared with those fed Escherichia coli OP50. The mean life span (determined as mean percent survival) of worms fed L. plantarum CJLP133 and L. fermentum LA12 was 13.89% and 13.69% greater, respectively, than that of control nematodes after 21. days (P=0.036 and 0.043, respectively). In addition, some of safety profiles, including hemolytic type, gelatin hydration and degradation of urea, were found to be positive. These newly identified lactobacilli hold promise for use as probiotic agents, feed additives and/or in food applications.
| Original language | English |
|---|---|
| Pages (from-to) | 80-86 |
| Number of pages | 7 |
| Journal | International Journal of Food Microbiology |
| Volume | 148 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2011 Aug 2 |
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Keywords
- C. elegans
- Immune modulation
- Lactobacilli
- Probiotic characteristics
ASJC Scopus subject areas
- Food Science
- Microbiology
- Safety, Risk, Reliability and Quality
Cite this
Evaluation of probiotic characteristics of newly isolated Lactobacillus spp. Immune modulation and longevity. / Lee, Jin; Yun, Hyun Sun; Cho, Kyu Won; Oh, Sejong; Kim, Sae Hun; Chun, Taehoon; Kim, Bongjoon; Whang, Kwang Youn.
In: International Journal of Food Microbiology, Vol. 148, No. 2, 02.08.2011, p. 80-86.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of probiotic characteristics of newly isolated Lactobacillus spp.
T2 - Immune modulation and longevity
AU - Lee, Jin
AU - Yun, Hyun Sun
AU - Cho, Kyu Won
AU - Oh, Sejong
AU - Kim, Sae Hun
AU - Chun, Taehoon
AU - Kim, Bongjoon
AU - Whang, Kwang Youn
PY - 2011/8/2
Y1 - 2011/8/2
N2 - In the current study, the probiotic potential of approximately 350 strains of lactic acid bacteria isolated from Korean infant feces and Kimchi was investigated. Common probiotic properties of the bacterial strains, such as acid tolerance, bile tolerance and adhesion to human intestinal epithelial cells (HT-29 cells), were examined. Some strains were found to have immune modulatory and antimicrobial properties. Antagonistic activity against a panel of pathogenic bacteria was found to be strain dependent. To evaluate the immune modulatory activity of the strains, lymphocyte interferon (IFN)-γ secretion was determined in conjunction with cell proliferation. Some strains of Lactobacillus gasseri, L. fermentum and L. plantarum exhibited increased IFN-γ levels and lymphocyte proliferation. To evaluate the effects of these immune modulating lactobacilli on host life span, Caenorhabditis elegans was used as an in vivo model. Nematodes that were supplied heat-killed lactobacilli as a food source exhibited obvious differences in life span compared with those fed Escherichia coli OP50. The mean life span (determined as mean percent survival) of worms fed L. plantarum CJLP133 and L. fermentum LA12 was 13.89% and 13.69% greater, respectively, than that of control nematodes after 21. days (P=0.036 and 0.043, respectively). In addition, some of safety profiles, including hemolytic type, gelatin hydration and degradation of urea, were found to be positive. These newly identified lactobacilli hold promise for use as probiotic agents, feed additives and/or in food applications.
AB - In the current study, the probiotic potential of approximately 350 strains of lactic acid bacteria isolated from Korean infant feces and Kimchi was investigated. Common probiotic properties of the bacterial strains, such as acid tolerance, bile tolerance and adhesion to human intestinal epithelial cells (HT-29 cells), were examined. Some strains were found to have immune modulatory and antimicrobial properties. Antagonistic activity against a panel of pathogenic bacteria was found to be strain dependent. To evaluate the immune modulatory activity of the strains, lymphocyte interferon (IFN)-γ secretion was determined in conjunction with cell proliferation. Some strains of Lactobacillus gasseri, L. fermentum and L. plantarum exhibited increased IFN-γ levels and lymphocyte proliferation. To evaluate the effects of these immune modulating lactobacilli on host life span, Caenorhabditis elegans was used as an in vivo model. Nematodes that were supplied heat-killed lactobacilli as a food source exhibited obvious differences in life span compared with those fed Escherichia coli OP50. The mean life span (determined as mean percent survival) of worms fed L. plantarum CJLP133 and L. fermentum LA12 was 13.89% and 13.69% greater, respectively, than that of control nematodes after 21. days (P=0.036 and 0.043, respectively). In addition, some of safety profiles, including hemolytic type, gelatin hydration and degradation of urea, were found to be positive. These newly identified lactobacilli hold promise for use as probiotic agents, feed additives and/or in food applications.
KW - C. elegans
KW - Immune modulation
KW - Lactobacilli
KW - Probiotic characteristics
UR - http://www.scopus.com/inward/record.url?scp=79959494621&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959494621&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2011.05.003
DO - 10.1016/j.ijfoodmicro.2011.05.003
M3 - Article
C2 - 21652104
AN - SCOPUS:79959494621
VL - 148
SP - 80
EP - 86
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
SN - 0168-1605
IS - 2
ER -