Evaluation of probiotic characteristics of newly isolated Lactobacillus spp. Immune modulation and longevity

Jin Lee, Hyun Sun Yun, Kyu Won Cho, Sejong Oh, Sae Hun Kim, Taehoon Chun, Bongjoon Kim, Kwang Youn Whang

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

In the current study, the probiotic potential of approximately 350 strains of lactic acid bacteria isolated from Korean infant feces and Kimchi was investigated. Common probiotic properties of the bacterial strains, such as acid tolerance, bile tolerance and adhesion to human intestinal epithelial cells (HT-29 cells), were examined. Some strains were found to have immune modulatory and antimicrobial properties. Antagonistic activity against a panel of pathogenic bacteria was found to be strain dependent. To evaluate the immune modulatory activity of the strains, lymphocyte interferon (IFN)-γ secretion was determined in conjunction with cell proliferation. Some strains of Lactobacillus gasseri, L. fermentum and L. plantarum exhibited increased IFN-γ levels and lymphocyte proliferation. To evaluate the effects of these immune modulating lactobacilli on host life span, Caenorhabditis elegans was used as an in vivo model. Nematodes that were supplied heat-killed lactobacilli as a food source exhibited obvious differences in life span compared with those fed Escherichia coli OP50. The mean life span (determined as mean percent survival) of worms fed L. plantarum CJLP133 and L. fermentum LA12 was 13.89% and 13.69% greater, respectively, than that of control nematodes after 21. days (P=0.036 and 0.043, respectively). In addition, some of safety profiles, including hemolytic type, gelatin hydration and degradation of urea, were found to be positive. These newly identified lactobacilli hold promise for use as probiotic agents, feed additives and/or in food applications.

Original languageEnglish
Pages (from-to)80-86
Number of pages7
JournalInternational Journal of Food Microbiology
Volume148
Issue number2
DOIs
Publication statusPublished - 2011 Aug 2

Fingerprint

Probiotics
Lactobacillus
probiotics
Modulation
Interferons
Lymphocytes
Bacteria
Food
HT29 Cells
Caenorhabditis elegans
interferons
Gelatin
Bile Acids and Salts
Feces
Urea
Lactic Acid
Cell proliferation
Lactic acid
Hot Temperature
Epithelial Cells

Keywords

  • C. elegans
  • Immune modulation
  • Lactobacilli
  • Probiotic characteristics

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Safety, Risk, Reliability and Quality

Cite this

Evaluation of probiotic characteristics of newly isolated Lactobacillus spp. Immune modulation and longevity. / Lee, Jin; Yun, Hyun Sun; Cho, Kyu Won; Oh, Sejong; Kim, Sae Hun; Chun, Taehoon; Kim, Bongjoon; Whang, Kwang Youn.

In: International Journal of Food Microbiology, Vol. 148, No. 2, 02.08.2011, p. 80-86.

Research output: Contribution to journalArticle

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