Evaluation of RGD-grafted porous PLLA scaffold for chondrogenic differentiation of stem cells

Hyun Jung, Kwideok Park, Kyoung Jin Cho, Kwang Duk Ahn, Dong June Ahn, Dong Keun Han

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Biodegradable porous poly(L-lactic acid) (PLLA) scaffolds were prepared using gas foaming method. The PLLA scaffolds with a hydrophobic surface were subjected to Ar plasma treatment and in situ acrylic acid (AA) grafting to obtain hydrophilic PLLA scaffold (PLLA-PAA). Cell-adhesive ROD peptide was then immobilized onto the AA-grafted PLLA (PLLA-PAA-RGD). Once rabbit bone marrow-derived mesenchymal stem cells (BM-MSC) were isolated, MSCs were seeded into PLLA control, PLLA-PAA, and PLLA-PAA-RGD scaffold and cultured for up to 4 weeks in chondrogenic medium with the addition of 10 ng/ml transforming growth factor (TGF)-β1. Surface analysis of AA-grafted PLLA identified significant alterations of surface characteristics, including reduced contact angle and different atomic compositions. From WST-1 assay at 4 weeks, cells were found more proliferative in PLLA-PAA than the others. Upon the histological analysis of Safranin O staining, chondrogenic differentiation of MSCs appeared to be progressed more actively in PLLA-PAA. The effect of RGD immobilization on MSC differentiation was barely notable.

Original languageEnglish
Pages (from-to)297-300
Number of pages4
JournalKey Engineering Materials
Volume342-343
DOIs
Publication statusPublished - 2007

Keywords

  • AA grafting
  • Chondrogenic differentiation
  • RGD
  • Scaffold
  • Stem cell
  • Surface modification
  • Tissue engineering

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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