Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[124I]iodobenzoate for cell tracking

Min Hwan Kim, Kyo Chul Lee, Gwang Il An, Sang Keun Woo, Noh Won Park, Byung Il Kim, Ki Dong Eom, Kwang Il Kim, Tae Sup Lee, Chan Wha Kim, Jeongsoo Yoo, Joo Hyun Kang, Yong Jin Lee

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2 Citations (Scopus)

Abstract

This study was aimed to evaluate the effect of 124I-labeling with hexadecyl-4-iodobenzoate (HIB) on gene expression related to cell cycle, DNA repair, transcription, proliferation and differentiation of adipose-derived stem cells (ADSCs). [124I]HIB showed high labeling efficiency with ADSCs (51.3±1.3%, 0.3-2.0Bq/cell) and there is no morphological change of ADSCs. In the microarray analysis of gene expression pattern, differences were not observed between non-labeled and [124I]HIB-labeled ADSCs. We demonstrated that 124I-labeling with HIB did not affect the biological properties of ADSCs.

Original languageEnglish
Pages (from-to)116-123
Number of pages8
JournalApplied Radiation and Isotopes
Volume108
DOIs
Publication statusPublished - 2016 Feb 1

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Keywords

  • Adipose-derived stem cells
  • Direct labeling agent
  • Hexadecyl-4-[I]iodobenzoate
  • Microarray
  • Myocardial infarction

ASJC Scopus subject areas

  • Radiation

Cite this

Kim, M. H., Lee, K. C., An, G. I., Woo, S. K., Park, N. W., Kim, B. I., Eom, K. D., Kim, K. I., Lee, T. S., Kim, C. W., Yoo, J., Kang, J. H., & Lee, Y. J. (2016). Evaluation of safety and efficacy of adipose-derived stem cells in rat myocardial infarction model using hexadecyl-4-[124I]iodobenzoate for cell tracking. Applied Radiation and Isotopes, 108, 116-123. https://doi.org/10.1016/j.apradiso.2015.12.039