Evaluation of the Abbott Cell-Dyn 4000 hematology analyzer for detection and therapeutic monitoring of Plasmodium vivax in the Republic of Korea

In Bum Suh, Hai Joong Kim, Jin Yong Kim, Sang Woo Lee, Seong Soo A An, Woo Joo Kim, Chae Seung Lim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The Cell-Dyn 4000 automated hematology analyzer (CD4000) has the ability to detect malaria patients, but it remained unclear whether it could detect persistent malaria post-treatment. To investigate this, we used the CD4000 to evaluate 68 Korean patients with Plasmodium vivax malaria, and control groups of 50 patients with fever and 50 asymptomatic patients. The results from the instrument-generated scatter plot (derived by laser light depolarization) were compared with microscopy results. During the initial diagnosis, the sensitivity of the CD4000 in detecting malaria was 91.2%. On day 3 of follow-up, the CD4000 results matched those from microscopy by 96.7%. Malaria was not detected by either method beyond 14 days post-presentation. Interestingly, the atypical depolarizing events, which typify the presence of malaria in the analyzer, were highly correlated with the levels of parasitaemia in serially diluted samples of the leucocyte-depleted blood, and the CD4000 detected parasites down to the level of 288 ± 17.7/μl. Our findings suggest that the phenomenon of atypical light depolarization could be influenced by parasitaemia levels, and be used as a screening method for P. vivax malaria patients, as well as for the therapeutic monitoring.

Original languageEnglish
Pages (from-to)1074-1081
Number of pages8
JournalTropical Medicine and International Health
Volume8
Issue number12
DOIs
Publication statusPublished - 2003 Dec 1

Fingerprint

Plasmodium vivax
Republic of Korea
Hematology
Malaria
Vivax Malaria
Parasitemia
Microscopy
Therapeutics
Light
Parasites
Lasers
Leukocytes
Fever
Control Groups

Keywords

  • Automated malaria detection
  • Cell-Dyn 4000
  • Korea
  • Plasmodium vivax

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases
  • Parasitology
  • Immunology

Cite this

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title = "Evaluation of the Abbott Cell-Dyn 4000 hematology analyzer for detection and therapeutic monitoring of Plasmodium vivax in the Republic of Korea",
abstract = "The Cell-Dyn 4000 automated hematology analyzer (CD4000) has the ability to detect malaria patients, but it remained unclear whether it could detect persistent malaria post-treatment. To investigate this, we used the CD4000 to evaluate 68 Korean patients with Plasmodium vivax malaria, and control groups of 50 patients with fever and 50 asymptomatic patients. The results from the instrument-generated scatter plot (derived by laser light depolarization) were compared with microscopy results. During the initial diagnosis, the sensitivity of the CD4000 in detecting malaria was 91.2{\%}. On day 3 of follow-up, the CD4000 results matched those from microscopy by 96.7{\%}. Malaria was not detected by either method beyond 14 days post-presentation. Interestingly, the atypical depolarizing events, which typify the presence of malaria in the analyzer, were highly correlated with the levels of parasitaemia in serially diluted samples of the leucocyte-depleted blood, and the CD4000 detected parasites down to the level of 288 ± 17.7/μl. Our findings suggest that the phenomenon of atypical light depolarization could be influenced by parasitaemia levels, and be used as a screening method for P. vivax malaria patients, as well as for the therapeutic monitoring.",
keywords = "Automated malaria detection, Cell-Dyn 4000, Korea, Plasmodium vivax",
author = "Suh, {In Bum} and Kim, {Hai Joong} and Kim, {Jin Yong} and Lee, {Sang Woo} and An, {Seong Soo A} and Kim, {Woo Joo} and Lim, {Chae Seung}",
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AU - Suh, In Bum

AU - Kim, Hai Joong

AU - Kim, Jin Yong

AU - Lee, Sang Woo

AU - An, Seong Soo A

AU - Kim, Woo Joo

AU - Lim, Chae Seung

PY - 2003/12/1

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