Evaluation of the bioaccumulation potential of selected alternative brominated flame retardants in marine fish using in vitro metabolic transformation rates

Hyun Jeoung Lee, Jee Hyun Jung, Jung-Hwan Kwon

Research output: Contribution to journalArticle

Abstract

The global consumption of alternative brominated flame retardants (BFRs) has increased with the restriction of the first generation BFRs such as polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecanes (HBCDs). However, many alternative BFRs are suspected to be persistent in the environment and possibly bioaccumulative after their release into the environment because of their chemical properties, which are similar to those of the already banned BFRs. In this study, the bioaccumulation potential of selected alternative BFRs (1,2-bis(2,4,6‑tribromophenoxy)ethane (BTBPE), 1,2,3,4,5,6‑hexabromobenzene (HBB), pentabromoethylbenzene (PBEB), 2,3,4,5,6‑pentabromotoluene (PBT), 2‑ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB), and 2,3,4,5‑tetrabromo-6-chlorotoluene (TBCT)) was evaluated. The in vitro depletion rate constants (kdepl) were measured for the alternative BFRs using liver S9 fractions isolated from five marine fish species (Epinephelus septemfasciatus, Konosirus punctatus, Lateolabrax japonicus, Mugil cephalus, and Sebastes schlegelii) that inhabit the oceans off the Korean coast. The measured kdepl values were converted to in vitro intrinsic clearance rate constants (CLin vitro) to estimate whole-body metabolic rate constants (kMET) using an in vitro to in vivo extrapolation (IVIVE) model. Finally, the bioconcentration factors (BCF) were determined using a one-compartment model. The transformation kinetics for obtaining kdepl agreed well with first-order chemical kinetics, regardless of initial BFR concentrations. The values of CLin vitro were lower in the selected marine fish species than those in freshwater fish species, implying slower metabolic transformation. The derived BCF values based on the total concentration in water (BCFTOT) ranged from 16 (TBB in M. cephalus) to 27,000 (HBB in K. punctatus). The BCF values for HBB and PBT were >2000 except for those in M. cephalus suggesting further investigation of BCF values of BFRs whose log KOW is between 6 and 7.

LanguageEnglish
Pages1333-1342
Number of pages10
JournalScience of the Total Environment
Volume653
DOIs
Publication statusPublished - 2019 Feb 25

Fingerprint

Flame Retardants
Bioaccumulation
Flame retardants
Fish
bioaccumulation
fish
Rate constants
Halogenated Diphenyl Ethers
kinetics
clearance rate
evaluation
flame retardant
rate
PBDE
ethane
Ethane
Extrapolation
Reaction kinetics
Liver
Chemical properties

Keywords

  • Alternatives
  • Bioconcentration
  • Biotransformation
  • Marine fish

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

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title = "Evaluation of the bioaccumulation potential of selected alternative brominated flame retardants in marine fish using in vitro metabolic transformation rates",
abstract = "The global consumption of alternative brominated flame retardants (BFRs) has increased with the restriction of the first generation BFRs such as polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecanes (HBCDs). However, many alternative BFRs are suspected to be persistent in the environment and possibly bioaccumulative after their release into the environment because of their chemical properties, which are similar to those of the already banned BFRs. In this study, the bioaccumulation potential of selected alternative BFRs (1,2-bis(2,4,6‑tribromophenoxy)ethane (BTBPE), 1,2,3,4,5,6‑hexabromobenzene (HBB), pentabromoethylbenzene (PBEB), 2,3,4,5,6‑pentabromotoluene (PBT), 2‑ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB), and 2,3,4,5‑tetrabromo-6-chlorotoluene (TBCT)) was evaluated. The in vitro depletion rate constants (kdepl) were measured for the alternative BFRs using liver S9 fractions isolated from five marine fish species (Epinephelus septemfasciatus, Konosirus punctatus, Lateolabrax japonicus, Mugil cephalus, and Sebastes schlegelii) that inhabit the oceans off the Korean coast. The measured kdepl values were converted to in vitro intrinsic clearance rate constants (CLin vitro) to estimate whole-body metabolic rate constants (kMET) using an in vitro to in vivo extrapolation (IVIVE) model. Finally, the bioconcentration factors (BCF) were determined using a one-compartment model. The transformation kinetics for obtaining kdepl agreed well with first-order chemical kinetics, regardless of initial BFR concentrations. The values of CLin vitro were lower in the selected marine fish species than those in freshwater fish species, implying slower metabolic transformation. The derived BCF values based on the total concentration in water (BCFTOT) ranged from 16 (TBB in M. cephalus) to 27,000 (HBB in K. punctatus). The BCF values for HBB and PBT were >2000 except for those in M. cephalus suggesting further investigation of BCF values of BFRs whose log KOW is between 6 and 7.",
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author = "Lee, {Hyun Jeoung} and Jung, {Jee Hyun} and Jung-Hwan Kwon",
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T1 - Evaluation of the bioaccumulation potential of selected alternative brominated flame retardants in marine fish using in vitro metabolic transformation rates

AU - Lee, Hyun Jeoung

AU - Jung, Jee Hyun

AU - Kwon, Jung-Hwan

PY - 2019/2/25

Y1 - 2019/2/25

N2 - The global consumption of alternative brominated flame retardants (BFRs) has increased with the restriction of the first generation BFRs such as polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecanes (HBCDs). However, many alternative BFRs are suspected to be persistent in the environment and possibly bioaccumulative after their release into the environment because of their chemical properties, which are similar to those of the already banned BFRs. In this study, the bioaccumulation potential of selected alternative BFRs (1,2-bis(2,4,6‑tribromophenoxy)ethane (BTBPE), 1,2,3,4,5,6‑hexabromobenzene (HBB), pentabromoethylbenzene (PBEB), 2,3,4,5,6‑pentabromotoluene (PBT), 2‑ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB), and 2,3,4,5‑tetrabromo-6-chlorotoluene (TBCT)) was evaluated. The in vitro depletion rate constants (kdepl) were measured for the alternative BFRs using liver S9 fractions isolated from five marine fish species (Epinephelus septemfasciatus, Konosirus punctatus, Lateolabrax japonicus, Mugil cephalus, and Sebastes schlegelii) that inhabit the oceans off the Korean coast. The measured kdepl values were converted to in vitro intrinsic clearance rate constants (CLin vitro) to estimate whole-body metabolic rate constants (kMET) using an in vitro to in vivo extrapolation (IVIVE) model. Finally, the bioconcentration factors (BCF) were determined using a one-compartment model. The transformation kinetics for obtaining kdepl agreed well with first-order chemical kinetics, regardless of initial BFR concentrations. The values of CLin vitro were lower in the selected marine fish species than those in freshwater fish species, implying slower metabolic transformation. The derived BCF values based on the total concentration in water (BCFTOT) ranged from 16 (TBB in M. cephalus) to 27,000 (HBB in K. punctatus). The BCF values for HBB and PBT were >2000 except for those in M. cephalus suggesting further investigation of BCF values of BFRs whose log KOW is between 6 and 7.

AB - The global consumption of alternative brominated flame retardants (BFRs) has increased with the restriction of the first generation BFRs such as polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecanes (HBCDs). However, many alternative BFRs are suspected to be persistent in the environment and possibly bioaccumulative after their release into the environment because of their chemical properties, which are similar to those of the already banned BFRs. In this study, the bioaccumulation potential of selected alternative BFRs (1,2-bis(2,4,6‑tribromophenoxy)ethane (BTBPE), 1,2,3,4,5,6‑hexabromobenzene (HBB), pentabromoethylbenzene (PBEB), 2,3,4,5,6‑pentabromotoluene (PBT), 2‑ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB), and 2,3,4,5‑tetrabromo-6-chlorotoluene (TBCT)) was evaluated. The in vitro depletion rate constants (kdepl) were measured for the alternative BFRs using liver S9 fractions isolated from five marine fish species (Epinephelus septemfasciatus, Konosirus punctatus, Lateolabrax japonicus, Mugil cephalus, and Sebastes schlegelii) that inhabit the oceans off the Korean coast. The measured kdepl values were converted to in vitro intrinsic clearance rate constants (CLin vitro) to estimate whole-body metabolic rate constants (kMET) using an in vitro to in vivo extrapolation (IVIVE) model. Finally, the bioconcentration factors (BCF) were determined using a one-compartment model. The transformation kinetics for obtaining kdepl agreed well with first-order chemical kinetics, regardless of initial BFR concentrations. The values of CLin vitro were lower in the selected marine fish species than those in freshwater fish species, implying slower metabolic transformation. The derived BCF values based on the total concentration in water (BCFTOT) ranged from 16 (TBB in M. cephalus) to 27,000 (HBB in K. punctatus). The BCF values for HBB and PBT were >2000 except for those in M. cephalus suggesting further investigation of BCF values of BFRs whose log KOW is between 6 and 7.

KW - Alternatives

KW - Bioconcentration

KW - Biotransformation

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