Evaluation of the compatibility of the Digene media when performing the Roche linear array human papillomavirus genotyping test

Jin Hwa Hong; Jae Kwan Lee; Eun Seop Song; Jong Min Lee; Mi Kyung Kim

doi:10.1007/s00404-009-0974-7

Evaluation of the compatibility of the Digene media when performing the Roche linear array human papillomavirus genotyping test

Jin Hwa Hong, Jae Kwan Lee, Eun Seop Song, Jong Min Lee, Mi Kyung Kim

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: This study was performed to evaluate the compatibility of the Digene media when performing the Roche linear array human papillomavirus (HPV) genotyping test. Methods: A total of 258 samples from 166 women were tested using the Hybrid Capture 2 (HC2) assay, the Cytyc media-based linear array test, and the Digene media-based linear array test. Results: The results between the HC2 assay and the Digene media-based linear array test were highly concordant (kappa = 0.78). The Cytyc media-based linear array test and Digene media-based linear array test exhibited substantial agreement in 207/249 cervical samples (kappa = 0.62). The two genotyping test also showed substantial agreement for the detection of HPV genotypes 16, 18, 52, 58 and for 18 HPV genotypes including both high-risk, and possible high-risk genotypes (kappa = 0.74, 0.69, respectively). Conclusions: We found Digene media to be interchangeable with Cytyc media when performing the Roche linear array genotyping test. This may be clinically meaningful in that we could perform the Roche linear array genotyping test with the same Digene media among women, positive for HC2 assay without the need for a return visit.

Original language	English
Pages (from-to)	613-618
Number of pages	6
Journal	Archives of Gynecology and Obstetrics
Volume	280
Issue number	4
DOIs	https://doi.org/10.1007/s00404-009-0974-7
Publication status	Published - 2009 Oct

Keywords

Digene media
Human papillomavirus
Linear array genotyping test

ASJC Scopus subject areas

Obstetrics and Gynaecology

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10.1007/s00404-009-0974-7

Cite this

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title = "Evaluation of the compatibility of the Digene media when performing the Roche linear array human papillomavirus genotyping test",

abstract = "Purpose: This study was performed to evaluate the compatibility of the Digene media when performing the Roche linear array human papillomavirus (HPV) genotyping test. Methods: A total of 258 samples from 166 women were tested using the Hybrid Capture 2 (HC2) assay, the Cytyc media-based linear array test, and the Digene media-based linear array test. Results: The results between the HC2 assay and the Digene media-based linear array test were highly concordant (kappa = 0.78). The Cytyc media-based linear array test and Digene media-based linear array test exhibited substantial agreement in 207/249 cervical samples (kappa = 0.62). The two genotyping test also showed substantial agreement for the detection of HPV genotypes 16, 18, 52, 58 and for 18 HPV genotypes including both high-risk, and possible high-risk genotypes (kappa = 0.74, 0.69, respectively). Conclusions: We found Digene media to be interchangeable with Cytyc media when performing the Roche linear array genotyping test. This may be clinically meaningful in that we could perform the Roche linear array genotyping test with the same Digene media among women, positive for HC2 assay without the need for a return visit.",

keywords = "Digene media, Human papillomavirus, Linear array genotyping test",

author = "Hong, {Jin Hwa} and Lee, {Jae Kwan} and Song, {Eun Seop} and Lee, {Jong Min} and Kim, {Mi Kyung}",

note = "Funding Information: This work was supported by a Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean government (MOST) (R01-2006-000-10621-0). We also thank Ms Young Mi Yun for her considerable devotion in statistical analysis of data. ",

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AU - Hong, Jin Hwa

AU - Lee, Jae Kwan

AU - Song, Eun Seop

AU - Lee, Jong Min

AU - Kim, Mi Kyung

N1 - Funding Information: This work was supported by a Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean government (MOST) (R01-2006-000-10621-0). We also thank Ms Young Mi Yun for her considerable devotion in statistical analysis of data.

PY - 2009/10

Y1 - 2009/10

N2 - Purpose: This study was performed to evaluate the compatibility of the Digene media when performing the Roche linear array human papillomavirus (HPV) genotyping test. Methods: A total of 258 samples from 166 women were tested using the Hybrid Capture 2 (HC2) assay, the Cytyc media-based linear array test, and the Digene media-based linear array test. Results: The results between the HC2 assay and the Digene media-based linear array test were highly concordant (kappa = 0.78). The Cytyc media-based linear array test and Digene media-based linear array test exhibited substantial agreement in 207/249 cervical samples (kappa = 0.62). The two genotyping test also showed substantial agreement for the detection of HPV genotypes 16, 18, 52, 58 and for 18 HPV genotypes including both high-risk, and possible high-risk genotypes (kappa = 0.74, 0.69, respectively). Conclusions: We found Digene media to be interchangeable with Cytyc media when performing the Roche linear array genotyping test. This may be clinically meaningful in that we could perform the Roche linear array genotyping test with the same Digene media among women, positive for HC2 assay without the need for a return visit.

AB - Purpose: This study was performed to evaluate the compatibility of the Digene media when performing the Roche linear array human papillomavirus (HPV) genotyping test. Methods: A total of 258 samples from 166 women were tested using the Hybrid Capture 2 (HC2) assay, the Cytyc media-based linear array test, and the Digene media-based linear array test. Results: The results between the HC2 assay and the Digene media-based linear array test were highly concordant (kappa = 0.78). The Cytyc media-based linear array test and Digene media-based linear array test exhibited substantial agreement in 207/249 cervical samples (kappa = 0.62). The two genotyping test also showed substantial agreement for the detection of HPV genotypes 16, 18, 52, 58 and for 18 HPV genotypes including both high-risk, and possible high-risk genotypes (kappa = 0.74, 0.69, respectively). Conclusions: We found Digene media to be interchangeable with Cytyc media when performing the Roche linear array genotyping test. This may be clinically meaningful in that we could perform the Roche linear array genotyping test with the same Digene media among women, positive for HC2 assay without the need for a return visit.

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Evaluation of the compatibility of the Digene media when performing the Roche linear array human papillomavirus genotyping test

Abstract

Keywords

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