Abstract
Purpose: This study was performed to evaluate the compatibility of the Digene media when performing the Roche linear array human papillomavirus (HPV) genotyping test. Methods: A total of 258 samples from 166 women were tested using the Hybrid Capture 2 (HC2) assay, the Cytyc media-based linear array test, and the Digene media-based linear array test. Results: The results between the HC2 assay and the Digene media-based linear array test were highly concordant (kappa = 0.78). The Cytyc media-based linear array test and Digene media-based linear array test exhibited substantial agreement in 207/249 cervical samples (kappa = 0.62). The two genotyping test also showed substantial agreement for the detection of HPV genotypes 16, 18, 52, 58 and for 18 HPV genotypes including both high-risk, and possible high-risk genotypes (kappa = 0.74, 0.69, respectively). Conclusions: We found Digene media to be interchangeable with Cytyc media when performing the Roche linear array genotyping test. This may be clinically meaningful in that we could perform the Roche linear array genotyping test with the same Digene media among women, positive for HC2 assay without the need for a return visit.
| Original language | English |
|---|---|
| Pages (from-to) | 613-618 |
| Number of pages | 6 |
| Journal | Archives of Gynecology and Obstetrics |
| Volume | 280 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2009 Oct 1 |
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Keywords
- Digene media
- Human papillomavirus
- Linear array genotyping test
ASJC Scopus subject areas
- Obstetrics and Gynaecology
Cite this
Evaluation of the compatibility of the Digene media when performing the Roche linear array human papillomavirus genotyping test. / Hong, Jin-Hwa; Lee, Jae Kwan; Song, Eun Seop; Lee, Jong Min; Kim, Mi Kyung.
In: Archives of Gynecology and Obstetrics, Vol. 280, No. 4, 01.10.2009, p. 613-618.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the compatibility of the Digene media when performing the Roche linear array human papillomavirus genotyping test
AU - Hong, Jin-Hwa
AU - Lee, Jae Kwan
AU - Song, Eun Seop
AU - Lee, Jong Min
AU - Kim, Mi Kyung
PY - 2009/10/1
Y1 - 2009/10/1
N2 - Purpose: This study was performed to evaluate the compatibility of the Digene media when performing the Roche linear array human papillomavirus (HPV) genotyping test. Methods: A total of 258 samples from 166 women were tested using the Hybrid Capture 2 (HC2) assay, the Cytyc media-based linear array test, and the Digene media-based linear array test. Results: The results between the HC2 assay and the Digene media-based linear array test were highly concordant (kappa = 0.78). The Cytyc media-based linear array test and Digene media-based linear array test exhibited substantial agreement in 207/249 cervical samples (kappa = 0.62). The two genotyping test also showed substantial agreement for the detection of HPV genotypes 16, 18, 52, 58 and for 18 HPV genotypes including both high-risk, and possible high-risk genotypes (kappa = 0.74, 0.69, respectively). Conclusions: We found Digene media to be interchangeable with Cytyc media when performing the Roche linear array genotyping test. This may be clinically meaningful in that we could perform the Roche linear array genotyping test with the same Digene media among women, positive for HC2 assay without the need for a return visit.
AB - Purpose: This study was performed to evaluate the compatibility of the Digene media when performing the Roche linear array human papillomavirus (HPV) genotyping test. Methods: A total of 258 samples from 166 women were tested using the Hybrid Capture 2 (HC2) assay, the Cytyc media-based linear array test, and the Digene media-based linear array test. Results: The results between the HC2 assay and the Digene media-based linear array test were highly concordant (kappa = 0.78). The Cytyc media-based linear array test and Digene media-based linear array test exhibited substantial agreement in 207/249 cervical samples (kappa = 0.62). The two genotyping test also showed substantial agreement for the detection of HPV genotypes 16, 18, 52, 58 and for 18 HPV genotypes including both high-risk, and possible high-risk genotypes (kappa = 0.74, 0.69, respectively). Conclusions: We found Digene media to be interchangeable with Cytyc media when performing the Roche linear array genotyping test. This may be clinically meaningful in that we could perform the Roche linear array genotyping test with the same Digene media among women, positive for HC2 assay without the need for a return visit.
KW - Digene media
KW - Human papillomavirus
KW - Linear array genotyping test
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UR - http://www.scopus.com/inward/citedby.url?scp=69249240195&partnerID=8YFLogxK
U2 - 10.1007/s00404-009-0974-7
DO - 10.1007/s00404-009-0974-7
M3 - Article
VL - 280
SP - 613
EP - 618
JO - Archives of Gynecology and Obstetrics
JF - Archives of Gynecology and Obstetrics
SN - 0932-0067
IS - 4
ER -