Evaluation of the effectiveness of various amendments on trace metals stabilization by chemical and biological methods

Sang Hwan Lee, Hyun Park, Namin Koo, Seunghun Hyun, Anna Hwang

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)


We evaluated the effects of five different kinds of amendments on heavy metals stabilization. The five amendments were: zero valent iron, limestone, acid mine drainage treatment sludge, bone mill, and bottom ash. To determine bioavailability of the heavy metals, different chemical extraction procedures were used such as, extraction with (Ca(NO3)2, DTPA; toxic characteristic leaching procedure (TCLP), physiologically based extraction test (PBET) that simulates gastric juice, and sequential extraction test. Bioavailability was also determined by measuring uptake of the heavy metals by lettuce (Lactuca sativa L.) and earthworms (Eisenia fetida). In addition, dehydrogenase activity was measured to determine microbial activity in the soil with the different amendments. The addition of amendments, especially limestone and bottom ash, resulted in a significant reduction in extractable metal contents. Biological assays using lettuce, earthworm, and enzyme activity were found as appropriate indicators of available metal fraction after in situ stabilization of heavy metals. In conclusion, TCLP and sequential extraction test appear to be promising surrogate measure of metal bioavailability in soils for several environment endpoints.

Original languageEnglish
Pages (from-to)44-51
Number of pages8
JournalJournal of hazardous materials
Issue number1-3
Publication statusPublished - 2011 Apr 15


  • Amendments
  • Bioaccessibility
  • Bioavailability
  • Heavy metals
  • Stabilization

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis


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