Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose

Jeong Do Kim, Byeong Jun Jeon, Jae Woo Han, Min Young Park, Sin Ae Kang, Beom Seok Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BACKGROUND: Endophytic bacteria are viewed as a potential new source of biofungicides because they have beneficial characteristics as control agents for plant disease. This study was performed to examine the endophytic feature and disease control efficacy of Bacillus amyloliquefaciens strain GYL4 and to identify the antifungal compounds produced by this strain. RESULTS: B. amyloliquefaciens strain GYL4 was isolated from leaf tissue of pepper plants (Capsicum annuum L.). Anthracnose symptoms were markedly reduced in the leaves of pepper plants colonised by GYL4. An egfp-expressing strain of GYL4 (GYL4-egfp) was constructed and reintroduced into pepper plants, which confirmed its ability to colonise the internal tissues of pepper plants. GYL4-egfp was observed in the root and stem tissues 4 days after treatment and abundantly found in the internal leaf tissue 9 days after treatment. Bacillomycin derivatives purified from the culture extract of GYL4 displayed control efficacy on anthracnose development in cucumber (Cucumis sativus L. cv. Chunsim). CONCLUSION: The present study is the first report on evaluation of the endophytic and systemic nature of B. amyloliquefaciens strain GYL4 and its potential as a biocontrol agent for anthracnose management.

Original languageEnglish
Pages (from-to)1529-1536
Number of pages8
JournalPest Management Science
Volume72
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1

Fingerprint

Bacillus amyloliquefaciens
anthracnose
pepper
leaves
biopesticides
Cucumis sativus
plant diseases and disorders
Capsicum annuum
signs and symptoms (plants)
cucumbers
biological control agents
disease control
chemical derivatives
stems
tissues
bacteria
extracts

Keywords

  • anthracnose
  • bacillomycin D
  • Bacillus amyloliquefaciens
  • biofungicide
  • endophytic bacteria
  • systemic colonisation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Insect Science

Cite this

Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose. / Kim, Jeong Do; Jeon, Byeong Jun; Han, Jae Woo; Park, Min Young; Kang, Sin Ae; Kim, Beom Seok.

In: Pest Management Science, Vol. 72, No. 8, 01.08.2016, p. 1529-1536.

Research output: Contribution to journalArticle

Kim, Jeong Do ; Jeon, Byeong Jun ; Han, Jae Woo ; Park, Min Young ; Kang, Sin Ae ; Kim, Beom Seok. / Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose. In: Pest Management Science. 2016 ; Vol. 72, No. 8. pp. 1529-1536.
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