Abstract
In this study, dilute sulfuric acid pretreatment was performed to improve the sugars recovery from Korean Miscanthus straw. The effect of pretreatment conditions on solubilized xylose was fundamentally investigated for the efficient removal of xylan. The optimal conditions were determined using a statistical method, and were shown to be a temperature of 121.6°C, an acid concentration of 1.1%, and a reaction time of 12.8 min. The combined severity factor was shown to be 1.1 under the optimum conditions. Following the pretreatment, the solubilized xylose in liquid fraction was found to be 71.2%, and about 72.6% of the solid was recovered. After enzymatic hydrolysis, about 86.4% glucose conversion was achieved when the pretreated biomass was used as a substrate, with the conversion being improved 4-fold compared with the control (untreated). The hydrolysates, approximately 10 g/L glucose, were applied to the fermentation of Saccharomyces cerevisiae K35, and the ethanol yield was about 96%. The overall process was evaluated based on the material balance, and the results show that approximately 172 g bioethanol can be produced when 1,000 g Miscanthus straw is loaded into the process.
| Original language | English |
|---|---|
| Pages (from-to) | 733-742 |
| Number of pages | 10 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 21 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2016 Nov 1 |
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Keywords
- bioethanol
- dilute acid pretreatment
- mass balance
- miscanthus
- process
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering
Cite this
Evaluation of the overall process on bioethanol production from miscanthus hydrolysates obtained by dilute acid pretreatment. / Yoo, Hah Young; Yang, Xiaoguang; Kim, Dong Sup; Lee, Soo Kweon; Lotrakul, Pongtharin; Prasongsuk, Sehanat; Punnapayak, Hunsa; Kim, Seung Wook.
In: Biotechnology and Bioprocess Engineering, Vol. 21, No. 6, 01.11.2016, p. 733-742.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the overall process on bioethanol production from miscanthus hydrolysates obtained by dilute acid pretreatment
AU - Yoo, Hah Young
AU - Yang, Xiaoguang
AU - Kim, Dong Sup
AU - Lee, Soo Kweon
AU - Lotrakul, Pongtharin
AU - Prasongsuk, Sehanat
AU - Punnapayak, Hunsa
AU - Kim, Seung Wook
PY - 2016/11/1
Y1 - 2016/11/1
N2 - In this study, dilute sulfuric acid pretreatment was performed to improve the sugars recovery from Korean Miscanthus straw. The effect of pretreatment conditions on solubilized xylose was fundamentally investigated for the efficient removal of xylan. The optimal conditions were determined using a statistical method, and were shown to be a temperature of 121.6°C, an acid concentration of 1.1%, and a reaction time of 12.8 min. The combined severity factor was shown to be 1.1 under the optimum conditions. Following the pretreatment, the solubilized xylose in liquid fraction was found to be 71.2%, and about 72.6% of the solid was recovered. After enzymatic hydrolysis, about 86.4% glucose conversion was achieved when the pretreated biomass was used as a substrate, with the conversion being improved 4-fold compared with the control (untreated). The hydrolysates, approximately 10 g/L glucose, were applied to the fermentation of Saccharomyces cerevisiae K35, and the ethanol yield was about 96%. The overall process was evaluated based on the material balance, and the results show that approximately 172 g bioethanol can be produced when 1,000 g Miscanthus straw is loaded into the process.
AB - In this study, dilute sulfuric acid pretreatment was performed to improve the sugars recovery from Korean Miscanthus straw. The effect of pretreatment conditions on solubilized xylose was fundamentally investigated for the efficient removal of xylan. The optimal conditions were determined using a statistical method, and were shown to be a temperature of 121.6°C, an acid concentration of 1.1%, and a reaction time of 12.8 min. The combined severity factor was shown to be 1.1 under the optimum conditions. Following the pretreatment, the solubilized xylose in liquid fraction was found to be 71.2%, and about 72.6% of the solid was recovered. After enzymatic hydrolysis, about 86.4% glucose conversion was achieved when the pretreated biomass was used as a substrate, with the conversion being improved 4-fold compared with the control (untreated). The hydrolysates, approximately 10 g/L glucose, were applied to the fermentation of Saccharomyces cerevisiae K35, and the ethanol yield was about 96%. The overall process was evaluated based on the material balance, and the results show that approximately 172 g bioethanol can be produced when 1,000 g Miscanthus straw is loaded into the process.
KW - bioethanol
KW - dilute acid pretreatment
KW - mass balance
KW - miscanthus
KW - process
UR - http://www.scopus.com/inward/record.url?scp=85009817175&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85009817175&partnerID=8YFLogxK
U2 - 10.1007/s12257-016-0485-x
DO - 10.1007/s12257-016-0485-x
M3 - Article
AN - SCOPUS:85009817175
VL - 21
SP - 733
EP - 742
JO - Biotechnology and Bioprocess Engineering
JF - Biotechnology and Bioprocess Engineering
SN - 1226-8372
IS - 6
ER -