Evaluation of the performance of a fecal tumor M2-PK rapid kit using stool specimens for detection of colorectal tumors

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Abstract

Background. In gastrointestinal tumors, only subunits of pyruvate kinase isoenzyme type M2 are detectable, and mainly in the dimeric form termed tumor M2-PK. A rapid test for the detection of M2-PK in stool has been developed. We evaluated the performance of the M2-PK rapid kit for the detection of colorectal tumors compared with colonoscopy. Design. Stool specimens (n=268) were obtained from patients who had a fecal occult blood test and colonoscopy performed for clinical evaluation or routine health checkup. Collected stool specimens were kept frozen at -20°C until testing. The fecal tumor M2-PK test was performed using a ScheBo M2-PK Quick test (ScheBo®•Biotech AG, Giessen, Germany). Results. A total of 236 patients were analyzed, including 87 with tubular adenoma and 126 controls. In tumor M2-PK testing, 37 of 87 tubular adenoma specimens (42.5%) and 2 of 4 sessile serrated adenoma specimens (50%) tested positive. The specificity of the M2-PK rapid test was 87.3%. Conclusion. The tumor M2-PK test demonstrated moderate sensitivity for detection of colorectal adenoma. M2-PK test could be considered as a useful point-of-care testing device for detection of colorectal tumors.

Original languageEnglish
Pages (from-to)154-160
Number of pages7
JournalAnnals of Clinical and Laboratory Science
Volume46
Issue number2
Publication statusPublished - 2016

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Keywords

  • Colonoscopy
  • Colorectal tumor
  • M2-PK
  • Rapid test
  • Stool

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Immunology
  • Microbiology
  • Hematology
  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

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