Evaluation of the plant growth-promoting activity of Pseudomonas nitroreducens in Arabidopsis thaliana and Lactuca sativa

Cao Son Trinh, Hyeri Lee, Won Je Lee, Seok Jin Lee, Namhyun Chung, Juhyeong Han, Jongyun Kim, Suk Whan Hong, Hojoung Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Key message: Pseudomonas nitroreducens: strain IHB B 13561 (PnIHB) enhances the growth of Arabidopsis thaliana and Lactuca sativa via the stimulation of cell development and nitrate absorption. Abstract: Plant growth-promoting rhizobacteria (PGPR) enhance plant development through various mechanisms; they improve the uptake of soil resources by plants to greatly promote plant growth. Here, we used Arabidopsis thaliana seedlings and Lactuca sativa to screen the growth enhancement activities of a purified PGPR, Pseudomonas nitroreducens strain IHB B 13561 (PnIHB). When cocultivated with PnIHB, both species of plants exhibited notably improved growth, particularly in regard to biomass. Quantitative reverse transcription polymerase chain reaction analysis indicated high expression levels of the nitrate transporter genes, especially NRT2.1, which plays a major role in the high-affinity nitrate transport system in roots. Moreover, enhanced activity of the cyclin-B1 promoter was observed when wild-type ‘Columbia-0’ Arabidopsis seedlings were exposed to PnIHB, whereas upregulation of cyclin-B also occurred in the inoculated lettuce seedlings. Overall, these results suggest that PnIHB improves A. thaliana and L. sativa growth via specific pathways involved in the promotion of cell development and enhancement of nitrate uptake.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalPlant Cell Reports
DOIs
Publication statusAccepted/In press - 2018 Mar 14

Fingerprint

Pseudomonas nitroreducens
Lactuca sativa
Arabidopsis thaliana
plant growth
nitrates
plant growth-promoting rhizobacteria
cyclins
seedlings
uptake mechanisms
soil resources
lettuce
transporters
plant development
quantitative polymerase chain reaction
reverse transcriptase polymerase chain reaction
promoter regions
Arabidopsis
cells
biomass

Keywords

  • Arabidopsis thaliana
  • CycB1
  • Lactuca sativa
  • NRT2
  • PGPR
  • PnIHB

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Evaluation of the plant growth-promoting activity of Pseudomonas nitroreducens in Arabidopsis thaliana and Lactuca sativa. / Trinh, Cao Son; Lee, Hyeri; Lee, Won Je; Lee, Seok Jin; Chung, Namhyun; Han, Juhyeong; Kim, Jongyun; Hong, Suk Whan; Lee, Hojoung.

In: Plant Cell Reports, 14.03.2018, p. 1-13.

Research output: Contribution to journalArticle

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