Evaluation of toxicological monitoring markers using proteomic analysis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In our study, we chose three different concentrations of FA (0, 5, and 10 ppm), and cytotoxic (lipid peroxidation and protein oxidation) and genotoxic assays (DNA damage) were carried out on plasma, blood, and liver cells of rats subjected to FA-inhalation treatment. The profiles of plasma protein changes determined using 2-DE analysis were also evaluated to identify potential toxicological monitoring markers in FA-exposed rats. Concern was raised that our genotoxic analyses did not follow previously published research data and that the results of our rat plasma proteomic studies were difficult to interpret because we did not directly determine the plasma concentration of FA. However, we had already determined the concentration of FA using HPLC in an exposure chamber to monitor FA inhalation concentrations. We suggest that our experimental design was suitable to determine the FA effects on rat using an inhalation chamber system. For the similarity of genotoxic effects in lymphocytes and liver cells, we chose to present our data on the general cytological toxic effects on lipid peroxidation and protein oxidation which revealed a similarity between plasma and liver cells of FA-exposed rats. We have shown strong correlations between genotoxicity and lipid peroxidation, and lipid peroxidation is known to mediate DNA damage in many in vitro, and in vivo studies. We are well aware of the 'implausibility' of leukemia induction by FA, but for precisely this reason, we feel the need for further study to prove the systemic genotoxic effects of FA.

Original languageEnglish
Pages (from-to)2525-2526
Number of pages2
JournalJournal of Proteome Research
Volume5
Issue number10
DOIs
Publication statusPublished - 2006 Oct 1

Fingerprint

Proteomics
Toxicology
Rats
Lipid Peroxidation
Monitoring
Liver
Inhalation
Lipids
Plasmas
DNA Damage
Oxidation
Lymphocytes
Poisons
DNA
Plasma Cells
Design of experiments
Blood Proteins
Assays
Blood Cells
Leukemia

Keywords

  • DNA damage
  • Formaldehyde
  • Lipid peroxidation
  • Protein oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics

Cite this

Evaluation of toxicological monitoring markers using proteomic analysis. / Sul, Dong Geun.

In: Journal of Proteome Research, Vol. 5, No. 10, 01.10.2006, p. 2525-2526.

Research output: Contribution to journalArticle

@article{2d6fbd6c325b497f859fc0736adaeb1f,
title = "Evaluation of toxicological monitoring markers using proteomic analysis",
abstract = "In our study, we chose three different concentrations of FA (0, 5, and 10 ppm), and cytotoxic (lipid peroxidation and protein oxidation) and genotoxic assays (DNA damage) were carried out on plasma, blood, and liver cells of rats subjected to FA-inhalation treatment. The profiles of plasma protein changes determined using 2-DE analysis were also evaluated to identify potential toxicological monitoring markers in FA-exposed rats. Concern was raised that our genotoxic analyses did not follow previously published research data and that the results of our rat plasma proteomic studies were difficult to interpret because we did not directly determine the plasma concentration of FA. However, we had already determined the concentration of FA using HPLC in an exposure chamber to monitor FA inhalation concentrations. We suggest that our experimental design was suitable to determine the FA effects on rat using an inhalation chamber system. For the similarity of genotoxic effects in lymphocytes and liver cells, we chose to present our data on the general cytological toxic effects on lipid peroxidation and protein oxidation which revealed a similarity between plasma and liver cells of FA-exposed rats. We have shown strong correlations between genotoxicity and lipid peroxidation, and lipid peroxidation is known to mediate DNA damage in many in vitro, and in vivo studies. We are well aware of the 'implausibility' of leukemia induction by FA, but for precisely this reason, we feel the need for further study to prove the systemic genotoxic effects of FA.",
keywords = "DNA damage, Formaldehyde, Lipid peroxidation, Protein oxidation",
author = "Sul, {Dong Geun}",
year = "2006",
month = "10",
day = "1",
doi = "10.1021/pr060384d",
language = "English",
volume = "5",
pages = "2525--2526",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Evaluation of toxicological monitoring markers using proteomic analysis

AU - Sul, Dong Geun

PY - 2006/10/1

Y1 - 2006/10/1

N2 - In our study, we chose three different concentrations of FA (0, 5, and 10 ppm), and cytotoxic (lipid peroxidation and protein oxidation) and genotoxic assays (DNA damage) were carried out on plasma, blood, and liver cells of rats subjected to FA-inhalation treatment. The profiles of plasma protein changes determined using 2-DE analysis were also evaluated to identify potential toxicological monitoring markers in FA-exposed rats. Concern was raised that our genotoxic analyses did not follow previously published research data and that the results of our rat plasma proteomic studies were difficult to interpret because we did not directly determine the plasma concentration of FA. However, we had already determined the concentration of FA using HPLC in an exposure chamber to monitor FA inhalation concentrations. We suggest that our experimental design was suitable to determine the FA effects on rat using an inhalation chamber system. For the similarity of genotoxic effects in lymphocytes and liver cells, we chose to present our data on the general cytological toxic effects on lipid peroxidation and protein oxidation which revealed a similarity between plasma and liver cells of FA-exposed rats. We have shown strong correlations between genotoxicity and lipid peroxidation, and lipid peroxidation is known to mediate DNA damage in many in vitro, and in vivo studies. We are well aware of the 'implausibility' of leukemia induction by FA, but for precisely this reason, we feel the need for further study to prove the systemic genotoxic effects of FA.

AB - In our study, we chose three different concentrations of FA (0, 5, and 10 ppm), and cytotoxic (lipid peroxidation and protein oxidation) and genotoxic assays (DNA damage) were carried out on plasma, blood, and liver cells of rats subjected to FA-inhalation treatment. The profiles of plasma protein changes determined using 2-DE analysis were also evaluated to identify potential toxicological monitoring markers in FA-exposed rats. Concern was raised that our genotoxic analyses did not follow previously published research data and that the results of our rat plasma proteomic studies were difficult to interpret because we did not directly determine the plasma concentration of FA. However, we had already determined the concentration of FA using HPLC in an exposure chamber to monitor FA inhalation concentrations. We suggest that our experimental design was suitable to determine the FA effects on rat using an inhalation chamber system. For the similarity of genotoxic effects in lymphocytes and liver cells, we chose to present our data on the general cytological toxic effects on lipid peroxidation and protein oxidation which revealed a similarity between plasma and liver cells of FA-exposed rats. We have shown strong correlations between genotoxicity and lipid peroxidation, and lipid peroxidation is known to mediate DNA damage in many in vitro, and in vivo studies. We are well aware of the 'implausibility' of leukemia induction by FA, but for precisely this reason, we feel the need for further study to prove the systemic genotoxic effects of FA.

KW - DNA damage

KW - Formaldehyde

KW - Lipid peroxidation

KW - Protein oxidation

UR - http://www.scopus.com/inward/record.url?scp=33750115019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750115019&partnerID=8YFLogxK

U2 - 10.1021/pr060384d

DO - 10.1021/pr060384d

M3 - Article

C2 - 17022623

AN - SCOPUS:33750115019

VL - 5

SP - 2525

EP - 2526

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 10

ER -