Evidence for both adenosine A1 and A2A receptors activating single vagal sensory C-fibres in guinea pig lungs

Benjamas Chuaychoo, Min-Goo Lee, Marian Kollarik, Rudolf Pullmann, Bradley J. Undem

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We addressed the hypothesis that single vagal afferent C-fibres can be stimulated via either the adenosine A1 or A2A receptor subtypes. The effect of adenosine on the nerve terminals of vagal sensory nerve subtypes was evaluated in an ex vivo perfused guinea pig lung preparation using extracellular recording techniques. Adenosine (10 μM) consistently evoked action potential discharge in lung C-fibre terminals arising from the nodose ganglia, but failed to evoke action potential discharge in most jugular ganglion C-fibres. Adenosine also failed to activate stretch-sensitive nodose A-fibres in the lungs. The selective A1 antagonist DPCPX (0.1 μM) or the selective A2A antagonist SCH 58261 (0.1 μM) partially inhibited the nodose C-fibre activation by adenosine, and the combination of both antagonists almost completely inhibited the response. The adenosine-induced action potential discharge in nodose C-fibres was mimicked by either the selective A1 agonist CCPA (1 μM) or the selective A2A agonist CGS 21680 (1 μM). Single cell PCR techniques revealed that adenosine A1 and A2A receptor mRNA was expressed in individual nodose neurons retrogradely labelled from the lungs. The gramicidin-perforated patch clamp technique on neurons retrogradely labelled from the lungs was employed to study the functional consequence of adenosine receptor agonists directly on neuronal membrane properties. Both the selective A1 agonist CCPA (1 μM) and the selective A2A agonist CGS 21680 (1 μM) depolarized the airway-specific, capsaicin-sensitive, nodose neurons to action potential threshold. The data support the hypothesis that adenosine selectively depolarizes vagal nodose C-fibre terminals in the lungs to action potential threshold, by stimulation of both adenosine A1 and A2A receptor subtypes located in the neuronal membrane. 2006 The Authors. Journal compilation

Original languageEnglish
Pages (from-to)481-490
Number of pages10
JournalJournal of Physiology
Volume575
Issue number2
DOIs
Publication statusPublished - 2006 Sep 1
Externally publishedYes

Fingerprint

Adenosine A2A Receptors
Adenosine A1 Receptors
Unmyelinated Nerve Fibers
Adenosine
Guinea Pigs
Action Potentials
Lung
Neurons
Nodose Ganglion
Purinergic P1 Receptor Agonists
Gramicidin
Myelinated Nerve Fibers
Membranes
Capsaicin
Patch-Clamp Techniques
Evoked Potentials
Ganglia
Neck
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Physiology

Cite this

Evidence for both adenosine A1 and A2A receptors activating single vagal sensory C-fibres in guinea pig lungs. / Chuaychoo, Benjamas; Lee, Min-Goo; Kollarik, Marian; Pullmann, Rudolf; Undem, Bradley J.

In: Journal of Physiology, Vol. 575, No. 2, 01.09.2006, p. 481-490.

Research output: Contribution to journalArticle

Chuaychoo, Benjamas ; Lee, Min-Goo ; Kollarik, Marian ; Pullmann, Rudolf ; Undem, Bradley J. / Evidence for both adenosine A1 and A2A receptors activating single vagal sensory C-fibres in guinea pig lungs. In: Journal of Physiology. 2006 ; Vol. 575, No. 2. pp. 481-490.
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