Evidence for multiple prolactin receptor transcripts in the turkey

G. R. Pitts, Seungkwon You, D. N. Foster, M. E. El Halawani

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Multiple prolactin receptor (PRL-R) mRNA transcript isoforms have been identified in mammals, but there are conflicting reports concerning the number of avian PRL-R isoforms. We hypothesized that multiple turkey PRL-R transcript isoforms exist and that PRL-R mRNA abundance may be related to reproductive status. Two turkey PRL-R cDNA fragments were generated using reverse transcriptase polymerase chain reaction (RT-PCR) that displayed a high degree of similarity to mammalian and avian PRL-R. Northern blot analysis of poly A+ mRNA hybridized to a turkey PRL-R riboprobe revealed a 3.1-kb band in the liver, oviduct, and testes. Additional 1.5- and 10.7-kb transcripts were found in the liver and testes, respectively. Hybridization of the same Northern blot to a chicken PRL-R probe verified the presence of a 3.1-kb transcript in all three tissues. A Northern blot was used to examine turkey PRL-R transcript isoform expression in laying hens. A 3.1-kb band was found in the pineal, infundibulum, magnum, isthmus, kidney, and intestine. In addition, 10.7- and 7.3-kb bands were detected in the pineal, magnum, isthmus, and intestine. Turkey PRL-R transcript isoforms were also examined throughout the reproductive cycle. The 10.7-, 7.3-, and 3.1-kb isoforms were detected in the oviduct, intestine, and pineal during each reproductive state. Turkey PRL-R mRNA levels were also compared during the reproductive cycle. Turkey PRL-R mRNA levels were greatest in laying hen pineal glands (P < 0.05) and in incubating hen oviducts. This study provides the first evidence for multiple PRL-R mRNA transcript isoforms in turkeys.

Original languageEnglish
Pages (from-to)355-362
Number of pages8
JournalPoultry Science
Volume79
Issue number3
Publication statusPublished - 2000 Mar 1
Externally publishedYes

Fingerprint

Prolactin Receptors
Protein Isoforms
Oviducts
Turkey
oviducts
Northern blotting
Northern Blotting
RNA Isoforms
Intestines
Messenger RNA
intestines
Turkey (country)
laying hens
prolactin receptors
Testis
testes
pineal body
liver
Pineal Gland
Liver

Keywords

  • mRNA
  • Prolactin receptor
  • Turkey

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Pitts, G. R., You, S., Foster, D. N., & El Halawani, M. E. (2000). Evidence for multiple prolactin receptor transcripts in the turkey. Poultry Science, 79(3), 355-362.

Evidence for multiple prolactin receptor transcripts in the turkey. / Pitts, G. R.; You, Seungkwon; Foster, D. N.; El Halawani, M. E.

In: Poultry Science, Vol. 79, No. 3, 01.03.2000, p. 355-362.

Research output: Contribution to journalArticle

Pitts, GR, You, S, Foster, DN & El Halawani, ME 2000, 'Evidence for multiple prolactin receptor transcripts in the turkey', Poultry Science, vol. 79, no. 3, pp. 355-362.
Pitts GR, You S, Foster DN, El Halawani ME. Evidence for multiple prolactin receptor transcripts in the turkey. Poultry Science. 2000 Mar 1;79(3):355-362.
Pitts, G. R. ; You, Seungkwon ; Foster, D. N. ; El Halawani, M. E. / Evidence for multiple prolactin receptor transcripts in the turkey. In: Poultry Science. 2000 ; Vol. 79, No. 3. pp. 355-362.
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