Abstract
Multiple prolactin receptor (PRL-R) mRNA transcript isoforms have been identified in mammals, but there are conflicting reports concerning the number of avian PRL-R isoforms. We hypothesized that multiple turkey PRL-R transcript isoforms exist and that PRL-R mRNA abundance may be related to reproductive status. Two turkey PRL-R cDNA fragments were generated using reverse transcriptase polymerase chain reaction (RT-PCR) that displayed a high degree of similarity to mammalian and avian PRL-R. Northern blot analysis of poly A+ mRNA hybridized to a turkey PRL-R riboprobe revealed a 3.1-kb band in the liver, oviduct, and testes. Additional 1.5- and 10.7-kb transcripts were found in the liver and testes, respectively. Hybridization of the same Northern blot to a chicken PRL-R probe verified the presence of a 3.1-kb transcript in all three tissues. A Northern blot was used to examine turkey PRL-R transcript isoform expression in laying hens. A 3.1-kb band was found in the pineal, infundibulum, magnum, isthmus, kidney, and intestine. In addition, 10.7- and 7.3-kb bands were detected in the pineal, magnum, isthmus, and intestine. Turkey PRL-R transcript isoforms were also examined throughout the reproductive cycle. The 10.7-, 7.3-, and 3.1-kb isoforms were detected in the oviduct, intestine, and pineal during each reproductive state. Turkey PRL-R mRNA levels were also compared during the reproductive cycle. Turkey PRL-R mRNA levels were greatest in laying hen pineal glands (P < 0.05) and in incubating hen oviducts. This study provides the first evidence for multiple PRL-R mRNA transcript isoforms in turkeys.
| Original language | English |
|---|---|
| Pages (from-to) | 355-362 |
| Number of pages | 8 |
| Journal | Poultry Science |
| Volume | 79 |
| Issue number | 3 |
| Publication status | Published - 2000 Mar 1 |
| Externally published | Yes |
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Keywords
- mRNA
- Prolactin receptor
- Turkey
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Evidence for multiple prolactin receptor transcripts in the turkey. / Pitts, G. R.; You, Seungkwon; Foster, D. N.; El Halawani, M. E.
In: Poultry Science, Vol. 79, No. 3, 01.03.2000, p. 355-362.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence for multiple prolactin receptor transcripts in the turkey
AU - Pitts, G. R.
AU - You, Seungkwon
AU - Foster, D. N.
AU - El Halawani, M. E.
PY - 2000/3/1
Y1 - 2000/3/1
N2 - Multiple prolactin receptor (PRL-R) mRNA transcript isoforms have been identified in mammals, but there are conflicting reports concerning the number of avian PRL-R isoforms. We hypothesized that multiple turkey PRL-R transcript isoforms exist and that PRL-R mRNA abundance may be related to reproductive status. Two turkey PRL-R cDNA fragments were generated using reverse transcriptase polymerase chain reaction (RT-PCR) that displayed a high degree of similarity to mammalian and avian PRL-R. Northern blot analysis of poly A+ mRNA hybridized to a turkey PRL-R riboprobe revealed a 3.1-kb band in the liver, oviduct, and testes. Additional 1.5- and 10.7-kb transcripts were found in the liver and testes, respectively. Hybridization of the same Northern blot to a chicken PRL-R probe verified the presence of a 3.1-kb transcript in all three tissues. A Northern blot was used to examine turkey PRL-R transcript isoform expression in laying hens. A 3.1-kb band was found in the pineal, infundibulum, magnum, isthmus, kidney, and intestine. In addition, 10.7- and 7.3-kb bands were detected in the pineal, magnum, isthmus, and intestine. Turkey PRL-R transcript isoforms were also examined throughout the reproductive cycle. The 10.7-, 7.3-, and 3.1-kb isoforms were detected in the oviduct, intestine, and pineal during each reproductive state. Turkey PRL-R mRNA levels were also compared during the reproductive cycle. Turkey PRL-R mRNA levels were greatest in laying hen pineal glands (P < 0.05) and in incubating hen oviducts. This study provides the first evidence for multiple PRL-R mRNA transcript isoforms in turkeys.
AB - Multiple prolactin receptor (PRL-R) mRNA transcript isoforms have been identified in mammals, but there are conflicting reports concerning the number of avian PRL-R isoforms. We hypothesized that multiple turkey PRL-R transcript isoforms exist and that PRL-R mRNA abundance may be related to reproductive status. Two turkey PRL-R cDNA fragments were generated using reverse transcriptase polymerase chain reaction (RT-PCR) that displayed a high degree of similarity to mammalian and avian PRL-R. Northern blot analysis of poly A+ mRNA hybridized to a turkey PRL-R riboprobe revealed a 3.1-kb band in the liver, oviduct, and testes. Additional 1.5- and 10.7-kb transcripts were found in the liver and testes, respectively. Hybridization of the same Northern blot to a chicken PRL-R probe verified the presence of a 3.1-kb transcript in all three tissues. A Northern blot was used to examine turkey PRL-R transcript isoform expression in laying hens. A 3.1-kb band was found in the pineal, infundibulum, magnum, isthmus, kidney, and intestine. In addition, 10.7- and 7.3-kb bands were detected in the pineal, magnum, isthmus, and intestine. Turkey PRL-R transcript isoforms were also examined throughout the reproductive cycle. The 10.7-, 7.3-, and 3.1-kb isoforms were detected in the oviduct, intestine, and pineal during each reproductive state. Turkey PRL-R mRNA levels were also compared during the reproductive cycle. Turkey PRL-R mRNA levels were greatest in laying hen pineal glands (P < 0.05) and in incubating hen oviducts. This study provides the first evidence for multiple PRL-R mRNA transcript isoforms in turkeys.
KW - mRNA
KW - Prolactin receptor
KW - Turkey
UR - http://www.scopus.com/inward/record.url?scp=0034145236&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034145236&partnerID=8YFLogxK
M3 - Article
C2 - 10735202
AN - SCOPUS:0034145236
VL - 79
SP - 355
EP - 362
JO - Poultry Science
JF - Poultry Science
SN - 0032-5791
IS - 3
ER -