Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain

Ho Sung Lee, Dai In Kang, Seung-Zhoo Yoon, Yeon Hee Ryu, Inhyung Lee, Hoon Gi Kim, Byung Cheon Lee, Ki Bog Lee

Research output: Contribution to journalArticle

Abstract

With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-weekold rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix.

Original languageEnglish
Pages (from-to)1101-1106
Number of pages6
JournalNeural Regeneration Research
Volume10
Issue number7
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Dura Mater
Blood Vessels
Brain
Chromium
Hematoxylin
Transmission Electron Microscopy
Phase-Contrast Microscopy
Cerebellum
Electrons
Staining and Labeling

Keywords

  • Brain
  • Chromium-hematoxylin staining
  • Dura mater
  • Fascia
  • Hormone
  • Nerve regeneration
  • Neural regeneration
  • Primo vascular system

ASJC Scopus subject areas

  • Developmental Neuroscience

Cite this

Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain. / Lee, Ho Sung; Kang, Dai In; Yoon, Seung-Zhoo; Ryu, Yeon Hee; Lee, Inhyung; Kim, Hoon Gi; Lee, Byung Cheon; Lee, Ki Bog.

In: Neural Regeneration Research, Vol. 10, No. 7, 01.01.2015, p. 1101-1106.

Research output: Contribution to journalArticle

Lee, Ho Sung ; Kang, Dai In ; Yoon, Seung-Zhoo ; Ryu, Yeon Hee ; Lee, Inhyung ; Kim, Hoon Gi ; Lee, Byung Cheon ; Lee, Ki Bog. / Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain. In: Neural Regeneration Research. 2015 ; Vol. 10, No. 7. pp. 1101-1106.
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