Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain

Ho Sung Lee, Dai In Kang, Seung Zhoo Yoon, Yeon Hee Ryu, Inhyung Lee, Hoon Gi Kim, Byung Cheon Lee, Ki Bog Lee

    Research output: Contribution to journalArticlepeer-review

    Abstract

    With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-weekold rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix.

    Original languageEnglish
    Pages (from-to)1101-1106
    Number of pages6
    JournalNeural Regeneration Research
    Volume10
    Issue number7
    DOIs
    Publication statusPublished - 2015 Jul 1

    Keywords

    • Brain
    • Chromium-hematoxylin staining
    • Dura mater
    • Fascia
    • Hormone
    • Nerve regeneration
    • Neural regeneration
    • Primo vascular system

    ASJC Scopus subject areas

    • Developmental Neuroscience

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