Evidence for the dimerization-mediated catalysis of methionine sulfoxide reductase a from Clostridium oremlandii

Eun Hye Lee, Kitaik Lee, Geun Hee Kwak, Yeon Seung Park, Kong Joo Lee, Kwang Yeon Hwang, Hwa Young Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Clostridium oremlandii MsrA (CoMsrA) is a natively selenocysteine-containing methionine-S-sulfoxide reductase and classified into a 1-Cys type MsrA. CoMsrA exists as a monomer in solution. Herein, we report evidence that CoMsrA can undergo homodimerization during catalysis. The monomeric CoMsrA dimerizes in the presence of its substrate methionine sulfoxide via an intermolecular disulfide bond between catalytic Cys16 residues. The dimeric CoMsrA is resolved by the reductant glutaredoxin, suggesting the relevance of dimerization in catalysis. The dimerization reaction occurs in a concentration- and time-dependent manner. In addition, the occurrence of homodimer formation in the native selenoprotein CoMsrA is confirmed. We also determine the crystal structure of the dimeric CoMsrA, having the dimer interface around the two catalytic Cys16 residues. A central cone-shaped hole is present in the surface model of dimeric structure, and the two Cys16 residues constitute the base of the hole. Collectively, our biochemical and structural analyses suggest a novel dimerization-mediated mechanism for CoMsrA catalysis that is additionally involved in CoMsrA regeneration by glutaredoxin.

Original languageEnglish
Article numbere0131523
JournalPLoS One
Volume10
Issue number6
DOIs
Publication statusPublished - 2015 Jun 24

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Clostridium
dimerization
Dimerization
Catalysis
catalytic activity
methionine
Glutaredoxins
Selenoproteins
Selenocysteine
selenocysteine
selenoproteins
methionine sulfoxide reductase
reducing agents
disulfide bonds
Reducing Agents
crystal structure
Disulfides
Methionine
Dimers
Cones

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Evidence for the dimerization-mediated catalysis of methionine sulfoxide reductase a from Clostridium oremlandii. / Lee, Eun Hye; Lee, Kitaik; Kwak, Geun Hee; Park, Yeon Seung; Lee, Kong Joo; Hwang, Kwang Yeon; Kim, Hwa Young.

In: PLoS One, Vol. 10, No. 6, e0131523, 24.06.2015.

Research output: Contribution to journalArticle

Lee, Eun Hye ; Lee, Kitaik ; Kwak, Geun Hee ; Park, Yeon Seung ; Lee, Kong Joo ; Hwang, Kwang Yeon ; Kim, Hwa Young. / Evidence for the dimerization-mediated catalysis of methionine sulfoxide reductase a from Clostridium oremlandii. In: PLoS One. 2015 ; Vol. 10, No. 6.
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