Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol

Hye Jin Lee, Soo Jung Kim, Jeong Jun Yoon, Kyoung Heon Kim, Jin Ho Seo, Yong Cheol Park

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The aim of this work was to apply the evolutionary engineering to construct a mutant Saccharomyces cerevisiae HJ7-14 resistant on 2-deoxy-d-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. In batch and repeated-batch fermentations, HJ7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental D452-2 strain. Transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief from the glucose-mediated repression of the transcription of the GAL genes might enable HJ7-14 to metabolize galactose rapidly. HJ7-14 produced 7.4 g/L ethanol from hydrolysates of the red alga Gelidium amansii within 12 h, which was 1.5-times faster than that observed with D452-2. We demonstrate conclusively that evolutionary engineering is a promising tool to manipulate the complex galactose metabolism in S. cerevisiae to produce bioethanol from red alga.

Original languageEnglish
Pages (from-to)445-451
Number of pages7
JournalBioresource Technology
Volume191
DOIs
Publication statusPublished - 2015 Jun 8

Keywords

  • Bioethanol
  • Evolutionary engineering
  • Galactose
  • Red algae
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Fingerprint Dive into the research topics of 'Evolutionary engineering of Saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol'. Together they form a unique fingerprint.

  • Cite this