Ex situ product recovery for enhanced butanol production by Clostridium beijerinckii

Sang Hyun Lee, Moon Ho Eom, Jin Dal Rae Choi, Sooah Kim, Jungyeon Kim, Yong An Shin, Kyoung Heon Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In situ butanol recovery fermentation has been intensively studied as an effective alternative to conventional butanol production, which is limited due to the cellular toxicity of butanol. However, the low biocompatibility of adsorbents often leads to failure of in situ recovery fermentations. In this study, Clostridium beijerinckii NCIMB 8052 was cultured in flasks without shaking and in situ recovery fermentation was performed by using an adsorbent L493. The amounts of acetone, butanol, and ethanol (ABE) increased by 34.4 % in the presence of the adsorbent. In contrast, cell growth and production of organic acids and ABE were retarded in the 7-L batch fermentations with in situ butanol recovery. Cell damage occurred in the fermentor upon agitation in the presence of the adsorbent, unlike in static flask cultures with in situ recovery. Ex situ recovery fermentation using circulation of fermentation broth after mid-exponential phase of cell growth was developed to avoid adsorbent-cell incompatibility. No apparent cell damage was observed and 25.7 g/L of ABE was produced from 86.2 g/L glucose in the fed-batch mode using 7 L fermentors. Thus, ex situ recovery fermentation with C. beijerinckii is effective for enhancing butanol fermentation.

Original languageEnglish
Pages (from-to)695-702
Number of pages8
JournalBioprocess and Biosystems Engineering
Volume39
Issue number5
DOIs
Publication statusPublished - 2016 May 1

Fingerprint

Clostridium beijerinckii
Clostridium
Butanols
Butenes
Fermentation
Recovery
Adsorbents
Acetone
Ethanol
Cell growth
Bioreactors
Cells
Organic acids
Growth
Biocompatibility
Glucose
Toxicity

Keywords

  • Adsorbent
  • Butanol
  • Clostridium beijerinckii
  • Ex situ butanol recovery

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Ex situ product recovery for enhanced butanol production by Clostridium beijerinckii. / Lee, Sang Hyun; Eom, Moon Ho; Choi, Jin Dal Rae; Kim, Sooah; Kim, Jungyeon; Shin, Yong An; Kim, Kyoung Heon.

In: Bioprocess and Biosystems Engineering, Vol. 39, No. 5, 01.05.2016, p. 695-702.

Research output: Contribution to journalArticle

Lee, Sang Hyun ; Eom, Moon Ho ; Choi, Jin Dal Rae ; Kim, Sooah ; Kim, Jungyeon ; Shin, Yong An ; Kim, Kyoung Heon. / Ex situ product recovery for enhanced butanol production by Clostridium beijerinckii. In: Bioprocess and Biosystems Engineering. 2016 ; Vol. 39, No. 5. pp. 695-702.
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