Exocas-2: Rapid and pure isolation of exosomes by anionic exchange using magnetic beads

Hyunsung Kim, Sehyun Shin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Extracellular vesicles (EVs) are considered essential biomarkers in liquid biopsies. Despite intensive efforts aimed at employing EVs in a clinical setting, workable approaches are currently limited owing to the fact that EV-isolation technologies are still in a nascent stage. This study introduces a magnetic bead-based ion exchange platform for isolating EVs called ExoCAS-2 (exosome clustering and scattering). Owing to their negative charge, exosomes can easily adhere to magnetic beads coated with a polycationic polymer. Owing to the features of magnetic beads, exosomes can be easily processed via washing and elution steps and isolated with high purity and yield within 40 min. The present results confirmed the isolation of exosomes through analyses of size distribution, morphology, surface and internal protein markers, and exosomal RNA. Compared with the commercially available methods, the proposed method showed superior performance in terms of key aspects, including operation time, purity, and recovery rate. This highlights the potential of this magnetic bead-based ion exchange platform for isolating exosomes present in blood plasma.

Original languageEnglish
Article number28
Pages (from-to)1-12
Number of pages12
JournalBiomedicines
Volume9
Issue number1
DOIs
Publication statusPublished - 2021

Keywords

  • Cationic polymer
  • Exosome
  • Ion exchange
  • Isolation
  • Magnetic beads

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

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