TY - JOUR
T1 - Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat
AU - Lee, Hong Sik
AU - Kim, Kwang Hee
AU - Kim, Chang Duck
AU - Song, Chi Wook
AU - Ryu, Ho Sang
AU - Hyun, Jin Hai
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1999/2
Y1 - 1999/2
N2 - To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with cannulation into bile duct, pancreatic duct, duodenum, and jugular vein and pancreatic juice was collected. For in vitro study, isolated pancreata of rats, pretreated with cysteamine, were perfused with an intraarterial infusion of Krebs-Henseleit solution (37°C) at 1.2 mL/min, and pancreatic juice was collected in 15-min samples. In vivo experiment of the rat, the mean basal pancreatic secretions, including volume, bicarbonate, and protein output were significantly increased from 18.4±0.5 μL/30 min, 0.58±0.05 μEq/30 min, and 214.0±26.1 μg/30 min to 51.6±3.7 μL/30 min, 1.52±0.11 μEq/30 min, and 569.8±128.9 μg/30 min, respectively (p<0.05). In the isolated perfused pancreas, cysteamine also resulted in a significant increase in basal pancreatic secretion (p<0.05). Simultaneous intraarterial infusion of octreotide (10 pmol/hr) to isolated pancreata partially reversed the effect of cysteamine on basal pancreatic secretion. These findings suggest that endogenous somatostatin play an important role on the regulation of basal pancreatic exocrine secretion.
AB - To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with cannulation into bile duct, pancreatic duct, duodenum, and jugular vein and pancreatic juice was collected. For in vitro study, isolated pancreata of rats, pretreated with cysteamine, were perfused with an intraarterial infusion of Krebs-Henseleit solution (37°C) at 1.2 mL/min, and pancreatic juice was collected in 15-min samples. In vivo experiment of the rat, the mean basal pancreatic secretions, including volume, bicarbonate, and protein output were significantly increased from 18.4±0.5 μL/30 min, 0.58±0.05 μEq/30 min, and 214.0±26.1 μg/30 min to 51.6±3.7 μL/30 min, 1.52±0.11 μEq/30 min, and 569.8±128.9 μg/30 min, respectively (p<0.05). In the isolated perfused pancreas, cysteamine also resulted in a significant increase in basal pancreatic secretion (p<0.05). Simultaneous intraarterial infusion of octreotide (10 pmol/hr) to isolated pancreata partially reversed the effect of cysteamine on basal pancreatic secretion. These findings suggest that endogenous somatostatin play an important role on the regulation of basal pancreatic exocrine secretion.
KW - Cysteamine
KW - Pancreatic function tests
KW - Rats
KW - Somatostatin
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U2 - 10.3346/jkms.1999.14.1.52
DO - 10.3346/jkms.1999.14.1.52
M3 - Article
C2 - 10102524
AN - SCOPUS:0033073783
VL - 14
SP - 52
EP - 56
JO - Journal of Korean Medical Science
JF - Journal of Korean Medical Science
SN - 1011-8934
IS - 1
ER -