Abstract
To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with cannulation into bile duct, pancreatic duct, duodenum, and jugular vein and pancreatic juice was collected. For in vitro study, isolated pancreata of rats, pretreated with cysteamine, were perfused with an intraarterial infusion of Krebs-Henseleit solution (37°C) at 1.2 mL/min, and pancreatic juice was collected in 15-min samples. In vivo experiment of the rat, the mean basal pancreatic secretions, including volume, bicarbonate, and protein output were significantly increased from 18.4±0.5 μL/30 min, 0.58±0.05 μEq/30 min, and 214.0±26.1 μg/30 min to 51.6±3.7 μL/30 min, 1.52±0.11 μEq/30 min, and 569.8±128.9 μg/30 min, respectively (p<0.05). In the isolated perfused pancreas, cysteamine also resulted in a significant increase in basal pancreatic secretion (p<0.05). Simultaneous intraarterial infusion of octreotide (10 pmol/hr) to isolated pancreata partially reversed the effect of cysteamine on basal pancreatic secretion. These findings suggest that endogenous somatostatin play an important role on the regulation of basal pancreatic exocrine secretion.
| Original language | English |
|---|---|
| Pages (from-to) | 52-56 |
| Number of pages | 5 |
| Journal | Journal of Korean Medical Science |
| Volume | 14 |
| Issue number | 1 |
| Publication status | Published - 1999 Feb 1 |
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Keywords
- Cysteamine
- Pancreatic function tests
- Rats
- Somatostatin
ASJC Scopus subject areas
- Medicine(all)
Cite this
Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat. / Lee, Hong Sik; Kim, Kwang Hee; Kim, Chang Duck; Song, Chi Wook; Ryu, Ho Sang; Hyun, Jin Hai.
In: Journal of Korean Medical Science, Vol. 14, No. 1, 01.02.1999, p. 52-56.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Exogenous cysteamine increases basal pancreatic exocrine secretion in the rat
AU - Lee, Hong Sik
AU - Kim, Kwang Hee
AU - Kim, Chang Duck
AU - Song, Chi Wook
AU - Ryu, Ho Sang
AU - Hyun, Jin Hai
PY - 1999/2/1
Y1 - 1999/2/1
N2 - To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with cannulation into bile duct, pancreatic duct, duodenum, and jugular vein and pancreatic juice was collected. For in vitro study, isolated pancreata of rats, pretreated with cysteamine, were perfused with an intraarterial infusion of Krebs-Henseleit solution (37°C) at 1.2 mL/min, and pancreatic juice was collected in 15-min samples. In vivo experiment of the rat, the mean basal pancreatic secretions, including volume, bicarbonate, and protein output were significantly increased from 18.4±0.5 μL/30 min, 0.58±0.05 μEq/30 min, and 214.0±26.1 μg/30 min to 51.6±3.7 μL/30 min, 1.52±0.11 μEq/30 min, and 569.8±128.9 μg/30 min, respectively (p<0.05). In the isolated perfused pancreas, cysteamine also resulted in a significant increase in basal pancreatic secretion (p<0.05). Simultaneous intraarterial infusion of octreotide (10 pmol/hr) to isolated pancreata partially reversed the effect of cysteamine on basal pancreatic secretion. These findings suggest that endogenous somatostatin play an important role on the regulation of basal pancreatic exocrine secretion.
AB - To determine whether exocrine pancreatic secretion is regulated by endogenous somatostatin, somatostatin deficiency was induced by cysteamine. Rats were subcutaneously administered a single dose of cysteamine (30 mg/100 g body weight) 12 hr before experiment. Anesthetized rats were prepared with cannulation into bile duct, pancreatic duct, duodenum, and jugular vein and pancreatic juice was collected. For in vitro study, isolated pancreata of rats, pretreated with cysteamine, were perfused with an intraarterial infusion of Krebs-Henseleit solution (37°C) at 1.2 mL/min, and pancreatic juice was collected in 15-min samples. In vivo experiment of the rat, the mean basal pancreatic secretions, including volume, bicarbonate, and protein output were significantly increased from 18.4±0.5 μL/30 min, 0.58±0.05 μEq/30 min, and 214.0±26.1 μg/30 min to 51.6±3.7 μL/30 min, 1.52±0.11 μEq/30 min, and 569.8±128.9 μg/30 min, respectively (p<0.05). In the isolated perfused pancreas, cysteamine also resulted in a significant increase in basal pancreatic secretion (p<0.05). Simultaneous intraarterial infusion of octreotide (10 pmol/hr) to isolated pancreata partially reversed the effect of cysteamine on basal pancreatic secretion. These findings suggest that endogenous somatostatin play an important role on the regulation of basal pancreatic exocrine secretion.
KW - Cysteamine
KW - Pancreatic function tests
KW - Rats
KW - Somatostatin
UR - http://www.scopus.com/inward/record.url?scp=0033073783&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033073783&partnerID=8YFLogxK
M3 - Article
C2 - 10102524
AN - SCOPUS:0033073783
VL - 14
SP - 52
EP - 56
JO - Journal of Korean Medical Science
JF - Journal of Korean Medical Science
SN - 1011-8934
IS - 1
ER -