Exon junction complex enhances translation of spliced mRNAs at multiple steps

Hyung Chul Lee, Junho Choe, Sung Gil Chi, Yoon Ki Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Translation of spliced mRNAs is enhanced by exon junction complex (EJC), which is deposited on mRNAs as a result of splicing. Although this phenomenon itself is well known, the underlying molecular mechanism remains poorly understood. Here we show, using siRNAs against Y14 and eIF4AIII and spliced or intronless constructs that contain different types of internal ribosome entry sites (IRESes), that Y14 and eIF4AIII increase translation of spliced mRNAs before and after formation of the 80S ribosome complex, respectively. These results suggest that EJC modulates translation of spliced mRNA at multiple steps.

Original languageEnglish
Pages (from-to)334-340
Number of pages7
JournalBiochemical and biophysical research communications
Volume384
Issue number3
DOIs
Publication statusPublished - 2009 Jul 3

Keywords

  • Exon junction complex
  • Internal ribosome entry site
  • Nonsense-mediated mRNA decay
  • Splicing
  • Y14
  • eIF4AIII

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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