Exosomal miRNA-19a and miRNA-614 induced by air pollutants promote proinflammatory m1 macrophage polarization via regulation of RORa expression in human respiratory mucosal microenvironment

Cheol Hee Shin, Junhyoung Byun, Kijeong Lee, Byoungjae Kim, Yong Kwan Noh, Na Ly Tran, Kwideok Park, Sang Heon Kim, Tae Hoon Kim, Seung Ja Oh

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Air pollution exposure leads to various inflammatory diseases in the human respiratory system. Chronic rhinosinusitis is an inflammatory disease caused by viruses, bacteria, or air pollutants. However, the underlying molecular mechanisms through which air particulate matter (PM) causes inflammation and disease remain unclear. In this article, we report that the induction of exosomal microRNAs (miRNAs) from human nasal epithelial cells upon airborne PM exposure promotes proinflammatory M1 macrophage polarization via downregulated RORa expression. Exposure of human nasal epithelial cells to PM results in inflammation-related miRNA expression, and more miRNA is secreted through exosomes delivered to macrophages. Among these, miRNA-19a and miRNA-614 directly bind to the 39-untranslated region of RORa mRNA and downregulate RORa expression, which leads to inflammation due to inflammatory cytokine upregulation and induces macrophages to a proinflammatory M1-like state. Finally, we showed enhanced expression of miRNA-19a and miRNA-614 but reduced RORa expression in a chronic rhinosinusitis patient tissue compared with the normal. Altogether, our results suggest that PM-induced exosomal miRNAs might play a crucial role in the proinflammatory mucosal microenvironment and macrophage polarization through the regulation of RORa expression.

Original languageEnglish
Pages (from-to)3179-3190
Number of pages12
JournalJournal of Immunology
Volume205
Issue number11
DOIs
Publication statusPublished - 2020 Dec 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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