Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration

Ji Suk Choi, Hwa In Yoon, Kyoung Soo Lee, Young Chan Choi, Seong Hyun Yang, In-San Kim, Yong Woo Cho

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Exosomes released from skeletal muscle cells play important roles in myogenesis and muscle development via the transfer of specific signal molecules. In this study, we investigated whether exosomes secreted during myotube differentiation from human skeletal myoblasts (HSkM) could induce a cellular response from human adipose-derived stem cells (HASCs) and enhance muscle regeneration in a muscle laceration mouse model. The exosomes contained various signal molecules including myogenic growth factors related to muscle development, such as insulin-like growth factors (IGFs), hepatocyte growth factor (HGF), fibroblast growth factor-2 (FGF2), and platelet-derived growth factor-AA (PDGF-AA). Interestingly, exosome-treated HASCs fused with neighboring cells at early time points and exhibited a myotube-like phenotype with increased expression of myogenic proteins (myosin heavy chain and desmin). On day 21, mRNAs of terminal myogenic genes were also up-regulated in exosome-treated HASCs. Moreover, in vivo studies demonstrated that exosomes from differentiating HSkM reduced the fibrotic area and increased the number of regenerated myofibers in the injury site, resulting in significant improvement of skeletal muscle regeneration. Our findings suggest that exosomes act as a biochemical cue directing stem cell differentiation and provide a cell-free therapeutic approach for muscle regeneration.

Original languageEnglish
Pages (from-to)107-115
Number of pages9
JournalJournal of Controlled Release
Volume222
DOIs
Publication statusPublished - 2016 Jan 28

Fingerprint

Exosomes
Muscle Development
Muscle Cells
Cues
Regeneration
Skeletal Muscle
Stem Cells
Skeletal Myoblasts
Skeletal Muscle Fibers
Muscles
Desmin
Hepatocyte Growth Factor
Myosin Heavy Chains
Lacerations
Platelet-Derived Growth Factor
Somatomedins
Fibroblast Growth Factor 2
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Phenotype

Keywords

  • Exosomes
  • Muscle regeneration
  • Myogenesis
  • Nanovesicles
  • Stem cells

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration. / Choi, Ji Suk; Yoon, Hwa In; Lee, Kyoung Soo; Choi, Young Chan; Yang, Seong Hyun; Kim, In-San; Cho, Yong Woo.

In: Journal of Controlled Release, Vol. 222, 28.01.2016, p. 107-115.

Research output: Contribution to journalArticle

Choi, Ji Suk ; Yoon, Hwa In ; Lee, Kyoung Soo ; Choi, Young Chan ; Yang, Seong Hyun ; Kim, In-San ; Cho, Yong Woo. / Exosomes from differentiating human skeletal muscle cells trigger myogenesis of stem cells and provide biochemical cues for skeletal muscle regeneration. In: Journal of Controlled Release. 2016 ; Vol. 222. pp. 107-115.
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