Expanded Polyglutamine Tract Itself Induces Cell Death in Cultured Cells

Kyoung Sook Bok, Hyangshuk Rhim, Young Do Yoo, Eui Ju Choi, Kwangseok Ahn, Ik Hwan Kim, Seong Man Kang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Several neurodegenerative diseases including Huntington disease, Machado-Joseph disease and spinocerebellar ataxias type 1 are caused by expansion of a polyglutamine tract within their respective gene products. In order to assess the role of the tract, 293T cells were transfected with plasmids that contain various lengths of CAG repeat encoding polyglutamine without the repeat disorder proteins: (CAG)27, (CAG)40, (CAG)80, (CAG)130, and (CAG)180. Except for (CAG)27, and (CAG)40, 293T cells showed a common set of morphological alterations such as shrinkage, rounding and surface blebbing when the expanded stretch was expressed. In addition, nuclear staining experiments showed chromatin condensation in COS-7 cells transfected with the vectors containing expanded CAG repeats. These results indicate that expanded polyglutamine itself is able to induce cell death, suggesting existence of a common molecular mechanism in the etiology of neurodegenerative polyglutamine diseases.

Original languageEnglish
Pages (from-to)398-402
Number of pages5
JournalMolecules and Cells
Volume9
Issue number4
Publication statusPublished - 1999 Aug 31

Fingerprint

Cultured Cells
Cell Death
HEK293 Cells
Neurodegenerative Diseases
Machado-Joseph Disease
Spinocerebellar Ataxias
COS Cells
Huntington Disease
Blister
Chromatin
Plasmids
Staining and Labeling
polyglutamine
Genes
Proteins

Keywords

  • Cell Death
  • Expansion
  • Polyglutamine

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Expanded Polyglutamine Tract Itself Induces Cell Death in Cultured Cells. / Bok, Kyoung Sook; Rhim, Hyangshuk; Yoo, Young Do; Choi, Eui Ju; Ahn, Kwangseok; Kim, Ik Hwan; Kang, Seong Man.

In: Molecules and Cells, Vol. 9, No. 4, 31.08.1999, p. 398-402.

Research output: Contribution to journalArticle

@article{d9120fbfb0964683920873835e8fedaa,
title = "Expanded Polyglutamine Tract Itself Induces Cell Death in Cultured Cells",
abstract = "Several neurodegenerative diseases including Huntington disease, Machado-Joseph disease and spinocerebellar ataxias type 1 are caused by expansion of a polyglutamine tract within their respective gene products. In order to assess the role of the tract, 293T cells were transfected with plasmids that contain various lengths of CAG repeat encoding polyglutamine without the repeat disorder proteins: (CAG)27, (CAG)40, (CAG)80, (CAG)130, and (CAG)180. Except for (CAG)27, and (CAG)40, 293T cells showed a common set of morphological alterations such as shrinkage, rounding and surface blebbing when the expanded stretch was expressed. In addition, nuclear staining experiments showed chromatin condensation in COS-7 cells transfected with the vectors containing expanded CAG repeats. These results indicate that expanded polyglutamine itself is able to induce cell death, suggesting existence of a common molecular mechanism in the etiology of neurodegenerative polyglutamine diseases.",
keywords = "Cell Death, Expansion, Polyglutamine",
author = "Bok, {Kyoung Sook} and Hyangshuk Rhim and Yoo, {Young Do} and Choi, {Eui Ju} and Kwangseok Ahn and Kim, {Ik Hwan} and Kang, {Seong Man}",
year = "1999",
month = "8",
day = "31",
language = "English",
volume = "9",
pages = "398--402",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "4",

}

TY - JOUR

T1 - Expanded Polyglutamine Tract Itself Induces Cell Death in Cultured Cells

AU - Bok, Kyoung Sook

AU - Rhim, Hyangshuk

AU - Yoo, Young Do

AU - Choi, Eui Ju

AU - Ahn, Kwangseok

AU - Kim, Ik Hwan

AU - Kang, Seong Man

PY - 1999/8/31

Y1 - 1999/8/31

N2 - Several neurodegenerative diseases including Huntington disease, Machado-Joseph disease and spinocerebellar ataxias type 1 are caused by expansion of a polyglutamine tract within their respective gene products. In order to assess the role of the tract, 293T cells were transfected with plasmids that contain various lengths of CAG repeat encoding polyglutamine without the repeat disorder proteins: (CAG)27, (CAG)40, (CAG)80, (CAG)130, and (CAG)180. Except for (CAG)27, and (CAG)40, 293T cells showed a common set of morphological alterations such as shrinkage, rounding and surface blebbing when the expanded stretch was expressed. In addition, nuclear staining experiments showed chromatin condensation in COS-7 cells transfected with the vectors containing expanded CAG repeats. These results indicate that expanded polyglutamine itself is able to induce cell death, suggesting existence of a common molecular mechanism in the etiology of neurodegenerative polyglutamine diseases.

AB - Several neurodegenerative diseases including Huntington disease, Machado-Joseph disease and spinocerebellar ataxias type 1 are caused by expansion of a polyglutamine tract within their respective gene products. In order to assess the role of the tract, 293T cells were transfected with plasmids that contain various lengths of CAG repeat encoding polyglutamine without the repeat disorder proteins: (CAG)27, (CAG)40, (CAG)80, (CAG)130, and (CAG)180. Except for (CAG)27, and (CAG)40, 293T cells showed a common set of morphological alterations such as shrinkage, rounding and surface blebbing when the expanded stretch was expressed. In addition, nuclear staining experiments showed chromatin condensation in COS-7 cells transfected with the vectors containing expanded CAG repeats. These results indicate that expanded polyglutamine itself is able to induce cell death, suggesting existence of a common molecular mechanism in the etiology of neurodegenerative polyglutamine diseases.

KW - Cell Death

KW - Expansion

KW - Polyglutamine

UR - http://www.scopus.com/inward/record.url?scp=0033620961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033620961&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 398

EP - 402

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 4

ER -