Expansion and deletion of CTG repeats from human disease genes are determined by the direction of replication in E. coli

Seongman Kang, Adam Jaworski, Keiichi Ohshima, Robert D. Wells

Research output: Contribution to journalArticle

304 Citations (Scopus)

Abstract

Several human hereditary neurological and neurodegenerative disease genes are associated with the expansion of CTG repeats. Here we show that the frequency of genetic expansions or deletions in Escherichia coli depends on the direction of replication. Large expansions occur predominantly when the CTGs are in the leading strand template rather than the lagging strand. However, deletions are more prominant when the CTGs are in the opposite orientation. Most deletions generated products of defined size classes. Strand slippage coupled with non–classical DMA structures may account for these observations and relate to expansion–deletion mechanisms in eukaryotic chromosomes for disease genes.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalNature Genetics
Volume10
Issue number2
DOIs
Publication statusPublished - 1995 Jun

ASJC Scopus subject areas

  • Genetics

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