@article{4096a0df42624fc0b3acba39cea66579,
title = "Expansion of the prime editing modality with Cas9 from Francisella novicida",
abstract = "Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace external bases in the target DNA sequence by linking reverse transcriptase to the Francisella novicida Cas9, which is a CRISPR-Cas9 ortholog. Using FnCas9(H969A) nickase, the targeting limitation of existing Streptococcus pyogenes Cas9 nickase [SpCas9(H840A)]-based prime editing is dramatically extended, and accurate prime editing is induced specifically for the target genes in human cell lines.",
keywords = "CRISPR-Cas9, Francisella novicida, Ortholog, Prime editing, Target expansion",
author = "Yeounsun Oh and Lee, {Wi jae} and Hur, {Junho K.} and Song, {Woo Jeung} and Youngjeon Lee and Hanseop Kim and Gwon, {Lee Wha} and Kim, {Young Hyun} and Park, {Young Ho} and Kim, {Chan Hyoung} and Lim, {Kyung Seob} and Song, {Bong Seok} and Huh, {Jae Won} and Kim, {Sun Uk} and Jun, {Bong Hyun} and Cheulhee Jung and Lee, {Seung Hwan}",
note = "Funding Information: The authors thank members of the National Primate Research Center (NPRC) for helpful discussions. Kevin Pang was the primary editor of this article and managed its editorial process and peer review in collaboration with the rest of the editorial team. The review history is available as Additional file 2. Funding Information: This research was supported by grants from the National Research Foundation funded by the Korean Ministry of Education, Science and Technology (NRF-2019R1C1C1006603, 2020R1I1A2075393, 2021M3A9I4024452); the Technology Innovation Program funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea) (20009707); the research fund of Hanyang University (HY-2020); the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (Project Number: 9991006929, KMDF_PR_20200901_ 0264); and KRIBB Research Initiative Program (KGM1051911, KGM4252122, KGM5382113, KGM4562121, KGM5282113). Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1186/s13059-022-02644-8",
language = "English",
volume = "23",
journal = "Genome Biology",
issn = "1474-7596",
publisher = "BioMed Central",
number = "1",
}