Experience on identification of cross-reactive group specificity performed by anti-human globulin panel reactive antibody tests

Yong Hak Sohn, Choong Hwan Cha, Myeong Hee Kim, Sun-Young Ko, Heung Bum Oh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background : Panel reactive antibody (PRA) is to screen and identify HLA antibody. Majority of antibody specificities in high-PRA are directed against cross reactive group (CREG). Thus, this study was to know the advantage of identifying CREG specificity and whether antibody specificities are changed according to CREG classification. Methods : HLA class I antibodies were identified from 159 sera from 108 patients in Asan Medical Center, who had shown more than 5% PRA by anti-human globulin (AHG)-complement-dependent cytotoxicity (CDC). Tail analysis-based computer program was developed to identify specificities, applying both Rodey (R-ABC) and Takemoto (T-ABC) classification. The results were also compared with those obtained when without CREG application (ABC). Results : Among 151 cases in which HLA specificities was identified, the frequency of CREG specificity was 22.5% in R-ABC and 27.2% in T-ABC. Eleven cases showed CREG specificities only in one classification. However, the individual antigen specificities in one hand were all included in the CREG identified in the other hand. CREG specificities in samples with PRA >50% (60%) were more frequently identified than those in samples with PRA ≤50% (9%) (in R-ABC, P<0.0001). Without applying CREG to interpretation, specificity was not identified in 9 cases. Conclusions : Application of CREG enhanced the rate of antibody identification. Antibody specificities of those cases where CREG specificities were different between Rodey and Takemoto classifications were almost the same when compared at the individual antigen level. Therefore, it was thought that it makes no difference to use any one of these two classifications in interpreting PRA.

Original languageEnglish
Pages (from-to)362-370
Number of pages9
JournalKorean Journal of Laboratory Medicine
Volume28
Issue number5
DOIs
Publication statusPublished - 2008 Dec 1
Externally publishedYes

Fingerprint

Globulins
Antibodies
Antibody Specificity
Hand
Antigens
Immunoglobulin Isotypes
Tail
Anti-Idiotypic Antibodies
Software
Cytotoxicity
Computer program listings
Serum

Keywords

  • Antibody specificity
  • Computer program
  • Cross reactive group (CREG)
  • Human leukocyte antigen
  • Panel reactive antibody
  • Tail analysis

ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry

Cite this

Experience on identification of cross-reactive group specificity performed by anti-human globulin panel reactive antibody tests. / Sohn, Yong Hak; Cha, Choong Hwan; Kim, Myeong Hee; Ko, Sun-Young; Oh, Heung Bum.

In: Korean Journal of Laboratory Medicine, Vol. 28, No. 5, 01.12.2008, p. 362-370.

Research output: Contribution to journalArticle

@article{694212a3181b4a4d9a405bd6fe231f97,
title = "Experience on identification of cross-reactive group specificity performed by anti-human globulin panel reactive antibody tests",
abstract = "Background : Panel reactive antibody (PRA) is to screen and identify HLA antibody. Majority of antibody specificities in high-PRA are directed against cross reactive group (CREG). Thus, this study was to know the advantage of identifying CREG specificity and whether antibody specificities are changed according to CREG classification. Methods : HLA class I antibodies were identified from 159 sera from 108 patients in Asan Medical Center, who had shown more than 5{\%} PRA by anti-human globulin (AHG)-complement-dependent cytotoxicity (CDC). Tail analysis-based computer program was developed to identify specificities, applying both Rodey (R-ABC) and Takemoto (T-ABC) classification. The results were also compared with those obtained when without CREG application (ABC). Results : Among 151 cases in which HLA specificities was identified, the frequency of CREG specificity was 22.5{\%} in R-ABC and 27.2{\%} in T-ABC. Eleven cases showed CREG specificities only in one classification. However, the individual antigen specificities in one hand were all included in the CREG identified in the other hand. CREG specificities in samples with PRA >50{\%} (60{\%}) were more frequently identified than those in samples with PRA ≤50{\%} (9{\%}) (in R-ABC, P<0.0001). Without applying CREG to interpretation, specificity was not identified in 9 cases. Conclusions : Application of CREG enhanced the rate of antibody identification. Antibody specificities of those cases where CREG specificities were different between Rodey and Takemoto classifications were almost the same when compared at the individual antigen level. Therefore, it was thought that it makes no difference to use any one of these two classifications in interpreting PRA.",
keywords = "Antibody specificity, Computer program, Cross reactive group (CREG), Human leukocyte antigen, Panel reactive antibody, Tail analysis",
author = "Sohn, {Yong Hak} and Cha, {Choong Hwan} and Kim, {Myeong Hee} and Sun-Young Ko and Oh, {Heung Bum}",
year = "2008",
month = "12",
day = "1",
doi = "10.3343/kjlm.2008.28.5.362",
language = "English",
volume = "28",
pages = "362--370",
journal = "Annals of Laboratory Medicine",
issn = "2234-3806",
publisher = "Seoul National University",
number = "5",

}

TY - JOUR

T1 - Experience on identification of cross-reactive group specificity performed by anti-human globulin panel reactive antibody tests

AU - Sohn, Yong Hak

AU - Cha, Choong Hwan

AU - Kim, Myeong Hee

AU - Ko, Sun-Young

AU - Oh, Heung Bum

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Background : Panel reactive antibody (PRA) is to screen and identify HLA antibody. Majority of antibody specificities in high-PRA are directed against cross reactive group (CREG). Thus, this study was to know the advantage of identifying CREG specificity and whether antibody specificities are changed according to CREG classification. Methods : HLA class I antibodies were identified from 159 sera from 108 patients in Asan Medical Center, who had shown more than 5% PRA by anti-human globulin (AHG)-complement-dependent cytotoxicity (CDC). Tail analysis-based computer program was developed to identify specificities, applying both Rodey (R-ABC) and Takemoto (T-ABC) classification. The results were also compared with those obtained when without CREG application (ABC). Results : Among 151 cases in which HLA specificities was identified, the frequency of CREG specificity was 22.5% in R-ABC and 27.2% in T-ABC. Eleven cases showed CREG specificities only in one classification. However, the individual antigen specificities in one hand were all included in the CREG identified in the other hand. CREG specificities in samples with PRA >50% (60%) were more frequently identified than those in samples with PRA ≤50% (9%) (in R-ABC, P<0.0001). Without applying CREG to interpretation, specificity was not identified in 9 cases. Conclusions : Application of CREG enhanced the rate of antibody identification. Antibody specificities of those cases where CREG specificities were different between Rodey and Takemoto classifications were almost the same when compared at the individual antigen level. Therefore, it was thought that it makes no difference to use any one of these two classifications in interpreting PRA.

AB - Background : Panel reactive antibody (PRA) is to screen and identify HLA antibody. Majority of antibody specificities in high-PRA are directed against cross reactive group (CREG). Thus, this study was to know the advantage of identifying CREG specificity and whether antibody specificities are changed according to CREG classification. Methods : HLA class I antibodies were identified from 159 sera from 108 patients in Asan Medical Center, who had shown more than 5% PRA by anti-human globulin (AHG)-complement-dependent cytotoxicity (CDC). Tail analysis-based computer program was developed to identify specificities, applying both Rodey (R-ABC) and Takemoto (T-ABC) classification. The results were also compared with those obtained when without CREG application (ABC). Results : Among 151 cases in which HLA specificities was identified, the frequency of CREG specificity was 22.5% in R-ABC and 27.2% in T-ABC. Eleven cases showed CREG specificities only in one classification. However, the individual antigen specificities in one hand were all included in the CREG identified in the other hand. CREG specificities in samples with PRA >50% (60%) were more frequently identified than those in samples with PRA ≤50% (9%) (in R-ABC, P<0.0001). Without applying CREG to interpretation, specificity was not identified in 9 cases. Conclusions : Application of CREG enhanced the rate of antibody identification. Antibody specificities of those cases where CREG specificities were different between Rodey and Takemoto classifications were almost the same when compared at the individual antigen level. Therefore, it was thought that it makes no difference to use any one of these two classifications in interpreting PRA.

KW - Antibody specificity

KW - Computer program

KW - Cross reactive group (CREG)

KW - Human leukocyte antigen

KW - Panel reactive antibody

KW - Tail analysis

UR - http://www.scopus.com/inward/record.url?scp=58149396327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149396327&partnerID=8YFLogxK

U2 - 10.3343/kjlm.2008.28.5.362

DO - 10.3343/kjlm.2008.28.5.362

M3 - Article

C2 - 18971617

AN - SCOPUS:58149396327

VL - 28

SP - 362

EP - 370

JO - Annals of Laboratory Medicine

JF - Annals of Laboratory Medicine

SN - 2234-3806

IS - 5

ER -