Experimental and computational characterization of biological liquid crystals: A review of single-molecule bioassays

Kilho Eom, Jaemoon Yang, Jinsung Park, Gwonchan Yoon, Soo Sohn Young, Shinsuk Park, Sung Yoon Dae, Sungsoo Na, Taeyun Kwon

Research output: Contribution to journalReview articlepeer-review

11 Citations (Scopus)

Abstract

Quantitative understanding of the mechanical behavior of biological liquid crystals such as proteins is essential for gaining insight into their biological functions, since some proteins perform notable mechanical functions. Recently, single-molecule experiments have allowed not only the quantitative characterization of the mechanical behavior of proteins such as protein unfolding mechanics, but also the exploration of the free energy landscape for protein folding. In this work, we have reviewed the current state-of-art in single-molecule bioassays that enable quantitative studies on protein unfolding mechanics and/or various molecular interactions. Specifically, single-molecule pulling experiments based on atomic force microscopy (AFM) have been overviewed. In addition, the computational simulations on single-molecule pulling experiments have been reviewed. We have also reviewed the AFM cantilever-based bioassay that provides insight into various molecular interactions. Our review highlights the AFM-based single-molecule bioassay for quantitative characterization of biological liquid crystals such as proteins.

Original languageEnglish
Pages (from-to)4009-4032
Number of pages24
JournalInternational journal of molecular sciences
Volume10
Issue number9
DOIs
Publication statusPublished - 2009 Sep

Keywords

  • Atomic force microscope (AFM)
  • Coarse-grained model
  • Coarse-grained molecular dynamics simulation
  • In vitro molecular recognitions
  • Single-molecule experiments

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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